复合添加剂缓解出栏肉牛运输应激效果评价  

Effects of anti-stress complex additive on beef cattle resistanting to stress

作　　者：连帅[1] 司鸿飞 王迪[1] 张婧馨 郭文晋[1] 甄莉[1] 杨焕民[1] 

机构地区：[1]黑龙江八一农垦大学动物科技学院,黑龙江大庆163319

出　　处：《中国兽医学报》2016年第9期1603-1607,共5页Chinese Journal of Veterinary Science

基　　金：公益性行业(农业)科研专项资助项目(2010030360-03-3)

摘　　要：为研究VC、VE、γ-氨基丁酸（γ-aminobutyric acid,GABA）、KHCO3抗应激复合添加剂对出栏肉牛抗应激的作用效果。选择16头健康、体况相近、体质量（430±20）kg的14月龄杂交肉牛,随机分为4组：A组（饲喂添加剂运输）、B组（饲喂添加剂不运输）、C组（不饲喂添加剂运输）、D组（不饲喂添加剂不运输）,A和B组每头牛添加VC、VE、GABA、KHCO3的剂量分别为4、2、1.2、6g/d,每日在饲料中添加1次,连续饲喂7d后进行8h、50~60km/h、2℃和相对湿度51%的公路运输,之后进行血清细胞因子、激素以及HSP70的检测。结果显示,运输应激后C组的IL-2浓度显著高于A、B和D三组（P〈0.05）;A和C组的IL-4、IL-10浓度显著低于B和D两组（P〈0.05）,A组与C组相比显著升高（P〈0.05）;A和C组的TNF-α浓度显著低于B和D两组（P〈0.05）;A和C组的血清中ACTH浓度显著高于B和D两组（P〈0.05）,A组与C组相比血清中ACTH浓度显著降低（P〈0.05）;C组的血清中GC浓度显著高于A、B和D三组（P〈0.05）;A和C组HSP70（热休克蛋白70）mRNA表达量显著高于B和C组（P〈0.05）,A组显著低于C组（P〈0.05）;A和C组HSP70蛋白显著高于B、D组（P〈0.05）。结果表明,8h、50~60km/h、2℃和相对湿度51%公路运输后能引起出栏肉牛出现应激反应,影响机体免疫系统;抗应激添加剂可以缓解出栏肉牛由运输引起的应激。This study was designend to evaluate the effect of anti-stress complex additive （VC,VE, GABA, KHCO3） on beef cattle resistanting to stress. Fourteen-months cross beef cattles（n = 16; BM： （430±20）kg） were randomly divided into four groups： A （complex additive with transported） ;B （complex additive without transported）;C（without complex additive but transported）;D （without complex additive and no transported）. First,each cattle in A and B groups was fed with 4 g VC,2 g VE, 1.2 g GABA,6 g KHCOa,once-daily for seven days, then, transported for eight hours. Last,cytokines,hormones and HSP70 were measured. The result indicated that IL-2 levels of group C were significantly higher（P（0.05）than that of groups A,B,and D;IL-4 and IL-10 levels of group A and C were significantly lower （P〈0.05）than that of groups B,and D,group A were significantly higher（P〈0.05）than that of group C;TNF-α levels of group A and C were significantly lower（P〈0.05）than that of groups B and D;on the contrary,ACTH levels of group A and C were significantly higher（P〈0.05）than that of groups B and D,while group A was signifi- cantly lower（P〈0.05） than that of groups C;GC levels of group C were significantly higher （P〈0.05）than that of groups B and D; HSP70 mRNA expression of group A and C was significantly higher than that of groups B and D,while group A was significantly lower（P〈0.05）than that of groups C;HSP70 protein expression of groups A and C was significantly higher（P〈0.05）than that of groups B and D. The results indicate that transportation for eight hours can cause beef cattle stress reaction,which affect the body immune system, while anti-stress additive can alleviate stress eqused by transportation.

关 键 词：运输应激 出栏肉牛 抗应激添加剂 HSP70 

分 类 号：S816.71[农业科学—饲料科学]