正交试验优选甘草制款冬花的炮制减毒工艺  

Optimization of Toxicity Attenuation Processing Technology for Farfarae Flos Being Processed with Glycyrrhizae Radix et Rhizoma by Orthogonal Test

作　　者：李明晓[1] 周臻[1,2] 田素英[1,2] 林剑峰[1] 

机构地区：[1]广东药科大学,广州510006 [2]广东省化妆品工程技术研究中心,广东中山528458

出　　处：《中国实验方剂学杂志》2016年第18期17-20,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：广东药学院大学生创新创业训练计划项目(201410573004)

摘　　要：目的:优选甘草制款冬花的炮制减毒工艺,为款冬花的炮制生产工艺提供参考。方法:以总生物碱含量为指标,甘草水煎煮次数、烘制温度及甘草用量(甘草质量/款冬花质量)为考察因素,采用正交试验对甘草制款冬花的减毒工艺进行优选,考察款冬花甘草制品中有效成分含量的变化。结果:款冬花甘草制最佳减毒工艺条件为甘草用量10%,水煎煮数3次,烘制温度90℃;甘草制款冬花中总生物碱、款冬酮及醇浸出物质量分数分别为0.080 3 mg·g-1,0.161%,26.31%。结论:与生品相比,款冬花甘草制品中总生物碱含量显著减少,款冬酮和醇浸出物含量增加。优选的款冬花甘草制减毒工艺稳定可行,提示甘草汁制款冬花有一定的合理性,具有实际应用意义。Objective： To optimize toxicity attenuation processing technology of Farfarae Flos being processed with Glycyrrhizae Radix et Rhizoma. Method： With the content of total alkaloids as index,toxicity attenuation processing technology of Farfarae Flos being processed with Glycyrrhizae Radix et Rhizoma was optimized by orthogonal test, and contents of alcohol-soluble extract and tussilagone in Farfarae Flos were determined before and after this optimized technology. Result： The best processing technology was as follows：adding 10% Glycyrrhizae Radix et Rhizoma,decocted three times,baking temperature at 90 ℃. Under these conditions,average content of total alkaloids was 0. 080 3 mg·g- 1,average content of tussilagone was 0. 161%,average mass fraction of alcohol-soluble extract was 26. 31%. Conclusion： After being processed,the content of total alkaloids in Farfarae Flos is decreased significantly,and contents of alcohol-soluble extract and tussilagone increase. This optimized processing technology is stable and feasible. This paper shows that Farfarae Flos being processed with Glycyrrhizae Radix et Rhizoma is rational.

关 键 词：款冬花 甘草制品 总生物碱 款冬酮 减毒工艺 

分 类 号：R283[医药卫生—中药学] R284.1[医药卫生—中医学]