玉屏风散加味方对慢性阻塞性肺疾病大鼠气道3种细菌的影响  

Effect of Modified Yupingfeng San on Three Kinds of Bacteria in Airway of COPD Rats

作　　者：彭静[1] 陈静[1] 田守征 邢海晶[1] 袁嘉丽[1] 

机构地区：[1]云南中医学院,昆明650500

出　　处：《中国实验方剂学杂志》2016年第18期123-126,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81460684)

摘　　要：目的:观察不同剂量玉屏风散加味方对慢性阻塞性肺疾病(COPD)大鼠气道流感嗜血杆菌、肺炎克雷伯菌、铜绿假单胞菌的影响,为玉屏风散加味方治疗COPD提供一定的实验依据。方法:Wistar大鼠通过气管滴注脂多糖(LPS)加烟熏的方法建立COPD大鼠模型,模型成功的大鼠随机分为模型组、玉屏风散加味方高、中、低剂量组(4.644,9.288,27.864 g·kg-1·d-1),另设正常组,每组10只。利用高通量测序分析每组3种细菌的含量,苏木素-伊红(HE)观察右肺组织病理学变化。结果:与正常组比较,模型组大鼠气道流感嗜血杆菌、肺炎克雷伯菌、铜绿假单胞菌含量明显升高(P<0.05),右肺组织病理学观察显示模型组大鼠右肺组织慢性炎症细胞增多和少量中性粒细胞浸润;与模型组比较,玉屏风散加味方低、中剂量组的流感嗜血杆菌含量均明显降低(P<0.05),玉屏风散加味方高、中、低剂量组的肺炎克雷伯菌、铜绿假单胞菌含量明显降低(P<0.05,P<0.01),大鼠右肺组织病变明显减轻。结论:玉屏风散加味方对COPD大鼠气道流感嗜血杆菌、肺炎克雷伯菌、铜绿假单胞菌含量有明显的降低作用,低剂量组对铜绿假单胞菌的作用更为明显,这可能是其治疗COPD的重要机制之一。Objective： To observe the effects of different doses of modified Yupingfeng San on Haemophilus influenza, Klebsiella pneumoniae and Pseudomonas aeruginosa in airway of chronic obstructive pulmonary disease（ COPD） rats, and provide experimental basis for the treatment of COPD by modified Yupingfeng San. Method： COPD rat models were induced by intratracheal instillation of LPS plus smoked method. The Wistar rats were randomly divided into normal group,model group,modified Yupingfeng San high dose,middle dose and low dose groups（ 4. 644,9. 288,27. 864 g·kg- 1·d- 1）,n = 10 in each group. The contents of 3 kinds of bacteria were analyzed by high-throughput sequencing method in each group,and the histopathological changes of the right lung were observed by HE staining. Result： As compared with the normal group,the contents of H. influenza,K. pneumoniae and P. aeruginosa were increased in the airway of model group mice（ P 0. 05）; right lung pathological observation results showed increase of chronic inflammatory cells in right lungs of model group and a small amount of neutrophil infiltration. As compared with the model group,the contents of H. influenza,K. pneumoniae and P. aeruginosa were decreased in modified Yupingfeng San low dose and middle dose groups（ P 0. 05）; the contents of the above bacteria were decreased in modified Yupingfeng San lowdose,middle dose and high dose groups（ P 0. 05,P 0. 01）,and the lesions in right lung tissues were significantly improved. Conclusion： Modified Yupingfeng San could significantly reduce the contents of H.influenza,K. pneumoniae and P. aeruginosa in airway of COPD rats,and the low dose group had more obvious effects on P. aeruginosa. This may be one of the important mechanisms for the treatment of COPD.

关 键 词：玉屏风散加味方 慢性阻塞性肺疾病 流感嗜血杆菌 肺炎克雷伯菌 铜绿假单胞菌 

分 类 号：R285.5[医药卫生—中药学]