响应面法优化狗枣猕猴桃叶粗多糖微波辅助提取工艺  

Optimization of the Microwave-assisted Extraction of Polysaccharide From the Leaves of Actinidia kolomikta(Rupr.et Maxim.)Planch Using Response Surface Methodology

作　　者：陆娟[1] 常清泉[1] 谭莉[1] 周洪玉 饶立东 

机构地区：[1]长春师范大学化学学院,吉林长春130032 [2]中节能万润股份有限公司,山东烟台264006

出　　处：《北方园艺》2016年第17期129-133,共5页Northern Horticulture

基　　金：吉林省教育厅"十二五"科学技术资助项目(吉教科合字[2013]第255号);长春师范大学自然科学基金资助项目(长师院自科合字政策[2010]第026号)

摘　　要：以狗枣猕猴桃叶为试材,在单因素试验的基础上,采用3因素3水平响应面分析法,研究了微波提取因素对狗枣猕猴桃叶粗多糖得率的影响,确定狗枣猕猴桃叶粗多糖微波提取工艺。结果表明:狗枣猕猴桃叶粗多糖微波最佳提取条件为微波提取时间44min,提取温度81℃,微波功率824W,在该条件下狗枣猕猴桃叶多糖的实际提取率可达6.72%,与理论的预测值6.82%的相对误差为0.1个百分点。Using the leaves of Actinidia kolomikta（Rupr.et Maxim.）Planch as test material,on the basis of single factor experiment,the effect of microwave-assisted extraction factors on crude polysaccharide from the leaves of Actinidia kolomikta（Rupr.et Maxim.）Planch were designed.A three-level-three-factor Box-Behnken design was employed to optimize the three extraction variables and to determine the optimum technology.The experimental results showed that the optimum technology was that the extraction time was 44 minutes,the extraction temperature was 81 ℃,the microwave power was 824 W Under the condition,the yield of the crude polysaccharide from the leaves of Actinidia kolomikta（Rupr.et Maxim.）Planch was 6.72%.The predictive value of the theory was 6.82%.The relative error was0.1percentage point.

关 键 词：狗枣猕猴桃 多糖 微波提取 响应面法 

分 类 号：Q946.33[生物学—植物学]