水稻粒簇生材料Cgr320的鉴定及遗传偏分离分析  

Observation and Genetic Segregation Distortion Analysis on Clustered Spikelets in Rice Resource Cgr320

作　　者：周军杰[1] 李兴星[1] 郑剑[1] 南文斌 秦小健[1] 张启军[2] 杨永清[1] 张汉马 梁永书[1,3] 

机构地区：[1]重庆师范大学生命科学学院/植物环境适应分子生物学重庆市重点实验室,重庆401331 [2]江苏省农业科学院粮食作物研究所,南京210014 [3]水稻生物学国家重点实验室,杭州310006

出　　处：《植物遗传资源学报》2016年第5期942-950,共9页Journal of Plant Genetic Resources

基　　金：国家“973”前期专项(2014CB160306);重庆师范大学博士启动基金(13XLB017);重庆市自然科学基金(cstc2014jcyj A80003);重庆市教委项目(KJ1400516)

摘　　要：籽粒簇生稻Cgr320为一类水稻突变材料，其性状表现为2—3朵颖花（籽粒）簇生在水稻主穗轴或枝梗顶部。为了进一步明确其簇生性状的遗传机制，本研究用Cgr320作父本分别与武运粳24和93—11配制了2个杂交组合，获得杂种F1、F2分离群体，对亲本、F1和F2群体的簇生性状进行了形态学观察和遗传连锁分析。结果表明，Cgr320其他农艺性状与普通栽培稻差异不显著。簇生性状在F1植株表现为野生型，在F2群体中出现严重偏离孟德尔（3：1）遗传分离，卡方测验值X（3：1）^2为7．71和144．87。随机选取第1、2、3、4、5、6、7、8、9、10、11和12染色体上RM493、RM3762、RM1338、RM3217、RM249、RM20155、RM3325、RM22418、RM6797、RM1146、RM7557和RM27706等12对微卫星标记对武运粳24／Cgr32022个F2隐性（簇生）单株进行遗传连锁分析，发现12个标记所扩增的22个F2隐性单株基因型都极显著偏向武运粳24，卡方检验值X（1：2：1）^2大于X（0.05,2）^2临界值5．991，控制cgr320簇生性状基因存在严重偏分离遗传，这种遗传现象必将误导我们判定控制籽粒簇生基因所在的连锁群。本研究结果将为水稻基因定位研究提供参考信息。Clustered grain rice （Cgr320）is a type of mutant material that clustered 2-3 grains on top of the main panicle. In order to clarify its genetic mechanism, we developed two F2 segregating populations derived from crosses between Cgr320 and WYJ24 or 93-11 and carried out the phenotypic observation experiment and analyses on genetic linkage of gene underlying Cgr320. The results showed that there were no significance differences in nine agronomical traits between Cgr320 and WYJ24 or 93-11 except clustered grain rice（Cgr） ,which showed recessive phenotype in F1 plants,indicating that the mutant is controlled by recessive gene. The segregation ration of mutant type plants to wild type plants in WYJ24/Cgr320 F2population was seriously deviated from the Mendel＇s laws of 3：1 ,the chi-square（χ^2 ） test values for Cgr genetic segregation were 7.71 and 144. 87, respectively. Twelve SSR markers distributing on rice chromosome 1,2,3,4,5,6,7,8,9,10,11 and 12 were used to perform analysis on genetic linkage of gene underlying Cgr using 22 F2 reeessive （mutant）plants, which showed serious segregation toward 2 WYJ24, and the chi-square（χ^2） test values for these markers were bigger than that of X（0.05,2）^25. 991, this will mis- lead us to judge the eorreet linkage group of gene controlling Cgr. To our knowledge, this is a rare ease of segregating distorting in rice gene mapping project analysis.

关 键 词：水稻 簇生稻 分子标记 遗传分析 偏分离 

分 类 号：S511[农业科学—作物学]