糖痹康对糖尿病周围神经病变大鼠坐骨神经PI3K/AKT信号通路的影响  

Influence of Tangbikang on PI3K/AKT signaling pathway of sciatic nerve in rats with diabetic peripheral neuropathy

作　　者：吕翠岩[1,2] 张胜容[1] 徐暾海[2] 孙文[2] 贾晓蕾[2] 赵文景[1] 张竹华[1] 刘铜华[2] 

机构地区：[1]首都医科大学附属北京中医医院,北京100010 [2]北京中医药大学,北京100029

出　　处：《中华中医药杂志》2016年第9期3710-3713,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家重大新药创制项目(No.2012ZX09102201-001);教育部博士点基金立项课题(No.20110013130001);北京中医药科技发展资金项目(No.JJ2015-06);北京中医药大学创新团队项目(No.2011-CXTD-19);教育部中医养生学重点实验室资助;北京市中医养生学重点实验室资助~~

摘　　要：目的:观察中药复方糖痹康对糖尿病大鼠坐骨神经AKT、P-AKT、P13K-P85、P-P13KP85表达的影响。方法:SD雄性大鼠60只,随机选取10只为正常组,其余大鼠采用高脂饲料和小剂量链脲佐菌素(STZ)诱发2型糖尿病大鼠动物模型。造模成功后随机分为模型组,甲钴胺组,糖痹康低、中、高剂量组,每组10只。各组采取相应干预。16周后取大鼠一侧坐骨神经,采用Western blot法检测大鼠坐骨神经AKT、P-AKT、P13K-P85、P-P13KP85的表达。结果:与正常组比较,模型组大鼠坐骨神经组织AKT、P-AKT、P13K-P85、P-P13KP85蛋白表达显著升高(P<0.01);与模型组比较,甲钴胺组及糖痹康低、中、高剂量组AKT、P-AKT、P13K-P85、P-P13KP85蛋白表达显著降低(P<0.01,P<0.05)。结论:抑制PI3K/AKT信号通路,可能是中药复方糖痹康防治DPN的作用机制之一。Objective：To observe Chinese herbal compound-Tangbikang on the expressions of AKT,P-AKT,P13K-P85 and P-P13KP85 of sciatic nerve in rats with diabetic peripheral neuropathy.Methods：Total of 60 male SD rats were involved this current study,where 10 rats were randomly selected to divide into control group,while the others were treated with high fat diet and a small dosage of streptozotocin injection to establish rat model of type 2 diabetes mellitus（T2DM）.The rats received modelling were randomly divided into model group,methycobal group,Tangbikang（low,medium and high dose） group,10 rats in each group.The expression of AKT,P-AKT,P13K-P85 and P-P13KP85 protein in unilateral sciatic nerve of 16 weeks were detected by Western blot.Results：Compared with the control group,the expression of AKT,P-AKT,P13K-P85 and P-P13KP85 protein in sciatic nerve were significantly increased in the model group（P〈0.01）.But,the expression of AKT,P-AKT,P13K-P85 and P-P13KP85 protein in sciatic nerve were significantly decreased in the methycobal group,Tangbikang（low,medium and high dose） group when compared to the model group（P〈0.01,P〈0.05）.Conclusion：Effects of Tangbikang on prevention and treatment of diabetic peripheral neuropathy may relate to inhibit PI3K/AKT signal pathway.

关 键 词：糖痹康 糖尿病周围神经病变 AKT P13K-P85 

分 类 号：R285.5[医药卫生—中药学]