半夏泻心汤含药血清对胃癌微环境诱导腹膜间皮HMrSV5细胞凋亡的影响  

Effects of drug serum of Banxia Xiexin Decoction on peritoneal mesothelial HMrSV5 cell apoptosis induced by gastric cancer microenvironment

作　　者：张炜[1] 刘喜平[2] 明海霞[2] 陈彦文[2] 李沛清[2] 吴高峰[2] 

机构地区：[1]兰州大学第一医院,兰州730000 [2]甘肃中医药大学基础医学院,兰州730000

出　　处：《中华中医药杂志》2016年第9期3735-3738,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.81260525);甘肃省高校基本科研业务费项目(No.BH-2011-068)~~

摘　　要：目的:观察半夏泻心汤含药血清对胃癌微环境诱导腹膜间皮细胞凋亡的抑制作用。方法:将胃癌BGC-823细胞上清液与人腹膜间皮HMr SV5细胞共培养,建立胃癌微环境对腹膜间皮细胞损伤模型,并以不同浓度的半夏泻心汤含药血清进行干预,倒置相差及荧光显微镜下观察腹膜间皮细胞细胞骨架及凋亡特征,Western blot法检测腹膜间皮细胞Bcl-2及Bax蛋白的表达。结果:经胃癌微环境诱导后腹膜间皮细胞脱落形成裸区,骨架稀疏,微丝变少、变细,散乱排列,并出现核溶解、碎裂、固缩等凋亡特征,Bcl-2蛋白表达下降(P<0.01),Bax蛋白表达增高(P<0.05),不同浓度的半夏泻心汤含药血清可保护腹膜间皮细胞细胞骨架,减少细胞凋亡,增加Bcl-2蛋白表达(P<0.05,P<0.01),降低Bax蛋白表达(P<0.05,P<0.01)。结论:半夏泻心汤含药血清能够抑制胃癌微环境诱导腹膜间皮细胞凋亡。Objective：To observe the inhibiting effect of drug serum of Banxia Xiexin Decoction on peritoneal mesothelial HMr SV5 cell apoptosis induced by gastric cancer microenvironment.Methods：Gastric cancer BGC-823 cellular supernatant and peritoneal mesothelium HMr SV5 cell were co-cultivate to establish damage model of gastric cancer microenvironment to peritoneal mesothelial cells.Intervene with different concentrations of drug serum of Banxia Xiexin Decoction,cytoskeleton and apoptosis characteristics of peritoneal mesothelial cells under l uorescence microscope were observed by inversion differ.The expression of Bcl-2 and Bax in peritoneal mesothelial cells were measured by Western blot method.Results：After being induced by gastric cancer microenvironment,the peritoneal mesothelial cells were fall of and form apterium presenting skeleton thinning,less and thinner microi lament,scattered arrangement,and with apoptosis characteristics such as nuclear dissolution,fracture,pyknosis.The protein expression of Bcl-2 was decrease（P〈0.01） while protein expression of Bax will increase（P〈0.05）.Dif erent concentrations of drug serum of Banxia Xiexin Decoction could protect cytoskeleton of peritoneal mesothelial cells,reduce apoptosis,increase Bcl-2 protein expression（P〈0.05,P〈0.01） and reduce Bax protein expression（P〈0.05,P〈0.01）.Conclusion：Drug serum of Banxia Xiexin Decoction can inhibit peritoneal mesothelial cells apoptosis induced by gstric cancer microenvironment.

关 键 词：胃癌 腹膜转移 胃癌微环境 腹膜间皮细胞 半夏泻心汤 

分 类 号：R285[医药卫生—中药学]