HPLC同时测定丹参多组分复合微乳中丹参酮ⅡA和丹酚酸B的含量  

Simultaneous determination of tanshinoneⅡ A and salvianolic acid B inmulti component compound microemulsion of Salvia miltiorrhiza Bge.by HPLC

作　　者：蒋俏丽 翟文文[1] 刘乐环 黄瑞雪[1] 戴俊东[1] 

机构地区：[1]北京中医药大学,北京100102

出　　处：《中华中医药杂志》2016年第9期3763-3765,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：北京中医药大学自主选题资助项目(No.2015-JYB-XS108)~~

摘　　要：目的:建立在同一色谱条件下同时测定丹参多组分复合微乳中丹参酮ⅡA和丹酚酸B的HPLC含量测定方法。方法:采用Kromasil C18(250mm×4.6mm,5μm)色谱柱,以乙腈-0.4%磷酸溶液(0-20min,10-45∶90-55;20-21min,45-80∶55-20;21-37min,80∶20;37-38min,80-10∶20-90,38-43min,10∶90)为流动相,流速1.2m L/min,丹参酮ⅡA和丹酚酸B的检测波长分别为270、286nm,进样量10μL。结果:丹参酮ⅡA和丹酚酸B分别在0.3-64μg/m L和10-210μg/m L范围内线性关系良好,加样回收率RSD均小于3%,以标示量计算的平均含量分别为(957.17±1.04)μg/g和(1 315.08±15.66)μg/g。结论:本方法简便、稳定、用时较少。该色谱条件下,各成分分离度良好,可用于丹参复合微乳的含量测定。Objective：To establish an HPLC method for the simultaneous determination of the contents of tanshinone ⅡA and salvianolic acid B in multicomponent compound microemulsion of Salvia miltiorrhiza Bge.under the same chromatographic condition.Methods：The determination was performed on a Kromasil C18 chromatographic column（250mm×4.6mm,5μm） with a mobile phase of acetonitrile-0.4% phosphate solution（0-20 min,10-45∶90-55;20-21 min,45-80∶55-20;21-37 min,80∶20;37-38 min,80-10∶20-90,38-43 min,10∶90）.The flow rate was 1.2m L/min,the detection wavelength of tanshinone II A and salvianolic acid B were 270 nm and 286 nm respectively.The injection volume was 10μL.Results：Good linear relationship between tanshinone II A and salvianolic acid B was achieved in the concentration of 0.3-64μg/m L and 10-210μg/m L respectively.The recovery RSD was less than 3%.The average contents of tanshinone II A and salvianolic acid B by indicated amount were（957.17±1.04） μg/g and（1 315.08±15.66） μg/g respectively.Conclusion：This method is simple,stable and time-saving,with a good resolution of each composition under this chromatographic condition.The method can be used for content determination of the compound microemulsion of Salvia miltiorrhiza Bge..

关 键 词：丹参 丹参酮ⅡA 丹酚酸B 微乳 高效液相色谱 含量测定 

分 类 号：R284.1[医药卫生—中药学] O657.72[医药卫生—中医学]