金线莲组培苗菌根化研究  

Study on mycorrhization of tissue-cultured Anoectochilus roxburghii

作　　者：陈雨薇[1] 唐军荣[1] 段雪甜 王国娟[1] 葛鸾 伍建榕[1] 

机构地区：[1]西南林业大学,昆明650224

出　　处：《中华中医药杂志》2016年第9期3778-3780,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：云南省教育厅科学研究基金资助项目(No.2013Y123);国家自然科学基金项目(No.31260175;No.31360198);云南省高校干热河谷植被恢复创新团队项目;云南省重点学科森林保护学项目(No.XKZ200905);云南省高等学校"森林病虫害综合治理"教学团队项目~~

摘　　要：目的:研究菌根真菌促进金线莲组培苗快速菌根化。方法:本研究从野生金线莲根中分离获得了16株菌根真菌,其中丝核菌类真菌有6株,根据菌丝形态特征将其合并为同一种(J8),经形态及分子鉴定为丝核菌属1种,将其与金线莲组培苗共生培养8个月后进行一系列的生物量测定、数据统计分析和石蜡切片观察。结果:组培苗与J8真菌共生后长势良好,鲜重增长率达70.8%,并从菌根的显微结构观察已形成菌丝结,证明组培苗已成功菌根化。结论:J8真菌的筛选为仿生野外种植金线莲提供理论依据。Objective：To promote the Anoectochilus roxburghifisomaclone mycorrhizal quickly.Methods：Isolated 16 strains mycorrhizal fungi from the root of wild Anoectochilus roxburghii,six strains belong to Rhizoctonia sp.,we combined them into the same kind（J8） according to the morphology of hyphae,after symbiotic culture with somaclone eight months,perform a series of biomass measurement,statistical analysis and parai n section observation.Results：The somaclone grew well after symbiotic with J8 fungus,fresh weight growth rate achieve 70.8%,and had formed hypha in the root,which proved that the somaclone had mycorrhizal successfully.Conclusion：The found of J8 provides theoretical basis for biomimetic field planting.

关 键 词：金线莲 组织培养 菌根真菌 菌根化 筛选 

分 类 号：S567.239[农业科学—中草药栽培]