液相色谱法测定婴幼儿食品包装材料中的脂肪胺  

Determination of Aliphatic Amines in Infant Food Packaging Materials by High Performance Liquid Chromatography

作　　者：盛存存 张世娟[1] 王芳芳[1] 纪仲胤[1] 窦昆[1] 尤进茂[1,2] 

机构地区：[1]山东省生命有机分析重点实验室,山东省绿色天然产物与医药中间体高校重点实验室,曲阜师范大学化学与化工学院,曲阜273165 [2]中国科学院西北高原生物研究所,西宁810001

出　　处：《分析化学》2016年第9期1365-1371,共7页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(No.21275089);山东省自然科学基金(No.ZR2014BQ009)资助~~

摘　　要：采用新型气流吹扫-微注射器萃取（GP-MSE）技术,用酸化甲醇为提取溶剂,对食品包装材料中脂肪胺进行了提取和富集。以10-乙基-吖啶酮-2-磺酰氯（EASC）为荧光试剂,在60℃、p H 10的条件下,对脂肪胺进行柱前荧光衍生,Hypersil GOLD柱分离,荧光检测的激发波长（λ（ex））和发射波长（λ（em））分别为262 nm和430 nm。12种脂肪胺检出限为0.4~0.6μg/kg,定量限为1.2~2.1μg/kg,在2.0~2000μg/L浓度范围内,线性相关系数均大于0.998。本方法具有快速、准确、灵敏的特点,用于婴幼儿食品包装材料分析,结果令人满意。Aliphatic amines in infant food packaging materials were extracted and concentrated by 0.5 m L of acidified methanol using gas purge microsyringe extraction（ GP-MSE）. Pre-column fluorescence labeling of amines was achieved in mild conditions with 10-ethyl-acridine-2-sulfonyl chloride（ EASC） as labeling reagent.The derivatization was carried out at 60℃ and p H 10. The derivatives were successfully separated on a Hypersil GOLD column with excitation and emission wavelengths of 262 and 430 nm,respectively. The detection limits were in the range of 0. 4- 0. 6 μg / kg,and the quantitation limits were in the range of 1. 2-2.1 μg / kg. All analytes were in good linearity in the concentration range of 2.0- 2000 μg / L with correlation coefficients of higher than 0. 998. The developed method was characterized by celerity,accuracy and high sensitivity. It was successfully applied to the determination of aliphatic amines in infant food packaging materials.

关 键 词：高效液相色谱法 气流吹扫-微注射器萃取技术 婴幼儿食品包装材料 荧光检测 

分 类 号：O657.72[理学—分析化学]