HPLC同时测定理气舒心片中3种活性成分  

Simultaneous determination of three active components in tablets of Liqishuxin with HPLC

作　　者：张静娇 李宪刚 鄢长余 

机构地区：[1]大连市药品检验所,辽宁大连116021

出　　处：《中国医药科学》2016年第15期44-46,共3页China Medicine And Pharmacy

摘　　要：目的利用HPLC同时测定理气舒心片中柚皮苷、橙皮苷和新橙皮苷。方法Thermo SCIENTIFIC Syncronis C18色谱柱（4．6mm×250mm，5μm）；流动相为乙腈-0．1％磷酸溶液（25：75）；流速1.0mL／min；柱温30℃；检测波长284nm。结果3种成分均达到基线分离，柚皮苷、橙皮苷和新橙皮苷的质量浓度与峰面积，分别在1．92～102．28μg／mL（r=0．9996），1．73～92．25μg／mL（r=0．9995），2．10～111．86μg／mL（r=0．9996）范围呈良好的线性关系；平均加样回收率分别为102．05％、99．53％、100．62％，RSD分别为0．89％、1．17％、2．02％。结论本研究建立的方法符合方法学验证要求，适用于同时测定理气舒心片中柚皮苷、橙皮苷和新橙皮苷的含量。Objective To simultaneously determine naringin,hesperidin and neohesperidin in tablets of Liqishuxin with HPLC. Methods The determination was performed by HPLC on Thermo SCIENTIFIC Syncronis C18 column （4.6mm × 250mm,5 μm）.The mobile phase consisted of acetonitrile-0.1% phosphoric acid solution（25：75） at a flow rate of 1.0 mL/min.The detection wavelength was set at 284 nm. Results Three active components were all in baseline separation.The calibration curves were linear in the range of 1.92-102.28 μg/mL for Naringin （r=0.9996）,1.73- 92.25 μg/mL for Hesperidin （r=0.9995）,2.10-111.86 μg/mL for Neohesperidin （r=0.9996）.Average rate of recoveries respectively were 102.05%,99.53%,100.62%,and RSD respectively were 0.89%,1.17% and 2.02%. Conclusion The method is consistent with the method validation requirements,and it applies for simultaneous determination of Naringin,Hesperidin and Neohesperidin three ingredients in tablets of Liqishuxin.

关 键 词：理气舒心片 柚皮苷 橙皮苷 新橙皮苷 HPLC 

分 类 号：R286.0[医药卫生—中药学]