黄连对白假丝酵母菌性阴道炎家兔阴道组织中TGF-β1表达的影响及临床意义  

Effect of Berberine on TGF-β1 Expression in Rabbits Model of Vulvovaginal Candidiasis and Clinical Significance

作　　者：张丽娟[1] 宁玉梅[1] 丁美红[2] 徐倩倩[1] 费霖莉[1] 李林彬[1] 

机构地区：[1]浙江中医药大学附属第二医院妇产科,浙江杭州310005 [2]浙江中医药大学药学院,浙江杭州310053

出　　处：《中华中医药学刊》2016年第9期2258-2260,I0012,共4页Chinese Archives of Traditional Chinese Medicine

基　　金：浙江省公益技术应用研究项目(2012C23077);浙江中医药科技计划重点项目(2012zz006)

摘　　要：目的:探讨黄连对家兔阴道组织中免疫状态的影响。方法:将雌激素化家兔随机分成黄连治疗组(A组)、克霉唑治疗组组(B组)、生理盐水组(C组),同时设未雌激素化未作任何处理的空白对照组(D组),除D组外在接种后第3、6、11天动态观察各组家兔阴道灌洗液中白假丝酵母菌菌丝生长情况,并在不同时间点处死家兔,摘取阴道组织,采用逆转录聚合酶链反应(RT-PCR)技术检测阴道组织中TGF-β1的表达变化。结果:接种后,A、B、C 3组阴道灌洗液均有菌丝及芽管形成;用药后,A、B组未见菌丝及芽管,仅见散在孢子,C组见大量菌丝生长、芽管形成;雌激素化后A、B、C三组阴道组织中TGF-β1表达水平均较D组明显升高(均P<0.05),但三组间无明显差异(均P>0.05)。用药后A组TGF-β1表达水平较B组、C组显著下降,差异有统计学意义(均P<0.01)。结论:黄连既能抑制白假丝酵母菌菌丝生长,又能下调阴道组织中TGF-β1的表达,改善阴道局部的免疫状态,可能是抑制白假丝酵母菌病发生及复发的重要原因,有望成为一种新的治疗复发性白假丝酵母菌病的靶向药物。Objective ： To investigate the effect of berberine on immune status of rabbit vaginal tissue. Methods ： The es- trogen - treated rabbits were divided into berberine group （ A）, clotrimazole group （B） and saline group （C） at random. The blank group（D） with no estrogen,no inoculated or no medicated was also set up. The hypha and germ tubes in the vaginal lavage fluid were observed at 3,6 and 11 days after inoculation. The rabbits were sacrificed at different time points,removing the vaginal tissue and using reverse transcription polymerase chain reaction （ RT - PCR） technique to detect expression changes of transforming growth factor - β1 （TGF - β1 ） in vaginal tissue. Results：After inoculation, hy- pha and germ tubes were seen in group A, B and C. After medication, no hypha or germ tube but scattered spores were seen in group A and B,while a great number of hypha and germ tube was seen in group C. After estrogen treatment, TGF - β1 expression levels of the vaginal mucosa of rabbits in group A,B and C were higher than that of group D（ all P 〈 0.05 ） but the difference among there groups were not statistically significant （ all P 〉 0.05 ）. After medication, com- pared with group B and C,TGF - β1 levels of group A declined significantly and the difference was statistically significant （ all P 〈 0. 01 ）. Conclusion：Berberine not only can inhibit the growth of Candida albicans hypha but also can lower TGF - β1 expression in vaginal tissues and improve vaginal immune status. This may be an important cause of berberine in- hibiting the recurrence of the disease. So berberine is expected to become a new targeted drug to the treatments of recur-rent candida albicans disease.

关 键 词：白假丝酵母菌病 外阴阴道 黄连 转化生长因子-Β1 

分 类 号：R285.5[医药卫生—中药学]