离子色谱-串联质谱法检测茶叶中的高氯酸盐  

Determination of perchlorates in tea by ion chromatographytandem mass spectrometry

作　　者：刘小芳[1] 方从容 刘慧 李仁勇 阎超[1] 

机构地区：[1]上海交通大学药学院,上海200240 [2]国家食品安全风险评估中心,北京100021 [3]赛默飞世尔科技(中国)有限公司,上海201206

出　　处：《色谱》2016年第10期986-988,共3页Chinese Journal of Chromatography

基　　金：国家自然科学基金项目(21175092);上海市科委科研计划项目(15142200200);上海市教育委员会科研创新项目(14YZ170);中国博士后科学基金资助项目(2015M581628)~~

摘　　要：建立了离子色谱-串联质谱检测茶叶中高氯酸盐的分析方法.选用高容量、强亲水性的IonPac AS2 0阴离子 交换柱（2 mm）进行分离,以淋洗液自动发生器在线产生的70 mmol/L 氢氧化钾为淋洗液等浓度淋洗, TSQ Quantiva三重四极杆质谱仪作检测器,采用电喷雾电离源负离子（ E S r ）模式、多反应监测（ MRM）模式进行分析, 内标法定量.结果表明,高氯酸盐在0. 02-10. 0 μg/L 范围内线性关系良好,相关系数（ r ）为 0. 999 1,定量限为2 μg/kg.运用该方法测定5种茶叶中的高氯酸盐,加标回收率为87.3%-112.2%.该方法具有操作简单、专属性 强、灵敏度高等优点,可满足茶叶中高氯酸盐的检测要求.A rapid ion chromatography-tandem mass spectrometry （IC-MS/MS） method was developed for the determination of perchlorates in tea. The chromatographic analysis was per-formed on Dionex 5000＋,using high capacity and hydroxide selective column IonPac AS20 （2 mm）. Perchlorates were separated by isocratic elution with 70 mmol/L KOH eluent generated from on-line eluent generation cartridge. TSQ Quantiva^TM,as a detector,scanned samples and monitored multi ion pairs simultaneously for confirmation and quantification,in the mode of negative ESI and multiple reaction monitoring （MRM）. The results displayed good linearity in the range of 0. 02-10. 0 μg/L,with correlation coefficient （r ） of 0. 999 1. The relative standard deviations （RSDs） were 2. 67% （n = 5）. The limits of quantification of the method were 2 μg/kg. The average recoveries of perchlorates in the five kinds of tea ranged from 87. 3% to 112. 2%. The results showed that the method is fast,simple,selective,sensitive and suitable for the determ nat on of perchlorates n var ous complex matr x samples such as tea.

关 键 词：离子色谱-串联质谱 高氯酸盐 茶叶 

分 类 号：O658[理学—分析化学]