牦牛HOXA1基因的时空表达及其对机体调控机制的研究  

Expression Pattern of HOXA1 Gene and Its Putative Regulating Mechanism of Yak

作　　者：张亚南[1] 石仙 熊显荣[1] 兰道亮[2] 李键[2] 

机构地区：[1]西南民族大学生命科学与技术学院,成都610041 [2]西南民族大学青藏高原研究院,成都610041

出　　处：《中国畜牧兽医》2016年第9期2265-2271,共7页China Animal Husbandry & Veterinary Medicine

基　　金：国家科技支撑计划(2012BAD13B06);国家公益性行业(农业)科研专项(201203009);西南民族大学研究生创新性项目(CX2015SZ068)

摘　　要：试验旨在获得牦牛HOXA1基因序列并进行生物信息学分析,同时分析其组织表达谱和时序表达谱,为进一步研究多脊椎骨形成的原因及其机制奠定基础。通过RT-PCR技术克隆多脊椎骨牦牛HOXA1基因,利用半定量RT-PCR技术检测该基因在多脊椎骨牦牛组织中的表达情况,利用实时荧光定量PCR技术检测该基因在多脊椎骨牦牛不同发育时期各组织中的表达情况。RT-PCR结果表明,多脊椎骨牦牛HOXA1基因序列全长为885bp,其中开放阅读框（ORF）为870bp,编码290个氨基酸。同源性分析表明,牦牛与普通牛、野牦牛、人、野猪、马、家鼠、黑猩猩、原鸡和野猪的同源性为75.4%~98.1%。组织表达分析表明,HOXA1基因在心脏、肝脏、脾脏、肺脏、肾脏、大肠、小肠、肌肉、胃、卵巢、子宫、输卵管、乳腺、睾丸中均有不同程度的表达;时序表达分析表明,随着年龄的增长HOXA1基因在多数组织中的表达量呈逐渐升高趋势。The aim of study was to obtain the yak HOXA1 gene sequence and related bioinformatics information,and analyze its expression spectrum and temporal expression profiles for providing related information of the formation of multiple vertebrae and its mechanism.RT-PCR technology was applied to clone the yak HOXA1 gene,semi-quantitative RT-PCR technique was used to detect the gene expression level in multiple organisms,and Real-time quantitative PCR technique was used to detect the gene expression level in the organisms in different development periods.The results showed that the obtained HOXA1 gene was 885 bp including the ORF of 870 bp,encoding 290 amino acids.The yak HOXA1 gene shared a homology of 75.4%to 98.1% with ordinary cow,wild yak,human,wild boar,horse,mice,chimpanzees,jungle fowl and wild boar.There were different expression level of HOXA1 gene in heart,liver,spleen,lung,kidney,large intestine,small intestine,muscle,stomach,ovaries,uterus,fallopian tubes,breast and testicular tissue.With the growth of the yak,HOXA1 gene expression level in tissue also increased.

关 键 词：牦牛 HOXA1基因 克隆 序列分析 组织表达 时序表达 

分 类 号：Q78[生物学—分子生物学]