肝癌肝移植术后肿瘤复发与外周血调节性T细胞及细胞因子表达水平的关系  

Correlation of tumor recurrence after liver transplantation for hepatocellular carcinoma with the expression levels of regulatory T cells and cytokines in peripheral blood

作　　者：杨绍臻 周林[2] 杜国盛[2] 宋继勇[2] 朱志东[2] 封立魁[2] 索龙龙[1] 郑永根 

机构地区：[1]河北北方学院 [2]解放军第309医院全军器官移植研究所肝胆外科 [3]海南五指山市75559部队医院

出　　处：《器官移植》2016年第5期382-385,共4页Organ Transplantation

基　　金：国家自然科学基金(81370578)

摘　　要：目的分析肝细胞癌(肝癌)肝移植术后肿瘤复发与调节性T细胞(Treg)及细胞因子表达水平的关系。方法以解放军第309医院2010年至2014年56例肝移植患者为研究对象。根据术后病理资料分为肝癌肝移植组(28例)和肝硬化肝移植组(肝硬化组,28例),肝癌肝移植组再根据术后患者有否肿瘤复发分为未复发组(8例)和复发组(20例)。比较各组患者外周血中Treg及调节性细胞因子[血管内皮生长因子(VEGF)、白细胞介素(IL)-2、IL-10、IL-12、转化生长因子(TGF)-β、干扰素(IFN)-γ]的表达水平。结果与肝硬化组比较,未复发组的IFN-γ、IL-12水平显著升高(均为P<0.05);复发组的Treg%、VEGF、IFN-γ、IL-10、TGF-β水平均显著升高,IL-2和IL-12水平明显降低(均为P<0.05)。与未复发组比较,复发组的Treg%、VEGF、IL-10、TGF-β水平均显著升高,IFN-γ、IL-2、IL-12水平明显降低(均为P<0.05)。结论Treg及细胞因子水平可作为肝癌肝移植术后肿瘤复发的预测指标。Objective To analyze the correlation of tumor recurrence after liver transplantation for hepatocellular carcinoma （HCC）with the expression levels of regulatory T cell （Treg）and cytokines in peripheral blood. Methods A total of 56 patients who underwent liver transplantation in the 309th Hospital of People’s Liberation Army from 2010 to 2014 were studied. According to the postoperative pathological data,all the patients were divided into the group of liver transplantation for HCC （HCC group,n=28）and group of liver transplantation for cirrhosis （liver cirrhosis group,n=28）, of which the HCC group was further divided into non-recurrence group （n=8）and recurrence group （n=20）according to the situation of postoperative tumor recurrence. The expression levels of Treg and cytokines [vascular endothelial growth factor （VEGF）,interleukin （IL）-2,IL-10,IL-12,transformation growth factor （TGF）-βand interferon （IFN）-γ]in peripheral blood of the patients in various groups were compared. Results Compared with the liver cirrhosis group,levels of IFN-γand IL-12 in the non-recurrence group increased significantly （both P〈0.05）;levels of Treg%,VEGF,IFN-γ, IL-10 and TGF-βin the recurrence group increased significantly,while levels of IL-2 and IL-12 decreased significantly （all P〈0.05）. Compared with the non-recurrence group,levels of Treg%,VEGF,IL-10 and TGF-βin the recurrence group increased significantly,while levels of IFN-γ,IL-2 and IL-12 decreased significantly （all P〈0.05 ）. Conclusions Levels of Treg and cytokines can be used to predict the tumor recurrence after liver transplantation for HCC.

关 键 词：肝移植 肿瘤复发 西罗莫司 调节性T细胞 细胞因子 

分 类 号：R617[医药卫生—外科学] R735.7[医药卫生—临床医学]