小麦脱氧雪腐镰刀菌烯醇(DON)快速筛查方法的研究  

Research on rapid screening method for deoxynivalenol( DON) in wheat

作　　者：欧阳毅[1] 唐芳[1] 张海洋[1] 祁智慧 程树峰[1] 

机构地区：[1]国家粮食局科学研究院,北京100037

出　　处：《粮油食品科技》2016年第5期73-76,共4页Science and Technology of Cereals,Oils and Foods

摘　　要：基于脱氧雪腐镰刀菌婦醇（DON ）产毒菌镰刀菌形态特征,以其大型分生孢子为检测目标 物,初步建立-种新的小麦DON 筛查方法.样品直接用水振荡提取,1 0 0目滤布过滤,滤液于显微 镜下镜检.检测时间约3 min.对不同感染水平的小麦阳性样品进行镰刀菌大孢子检验（ n = 6 ）, 方法相对标准偏差（RSD ）为7. 1%-21.6%.对我国小麦主产区的72个小麦样品进行检验,用 HPLC法作对照.结果表明,样品中有46个未检出镰刀菌大孢子,占样品总数的63.9 % ,这些样 品 DON 含量为0-792.4μ/kg,均未超过国家限量标准1 0 0 0μ/kg;有 2 6个样品检出大孢子, DON 未超标为6个,但均有检出,其余大部分样品镰刀菌生长量与其产生D O N 的产量呈正相关. 由此可见,通过本方法,可快速的对批量小麦DON 超标与未超标样品进行筛查区分.A new deoxynivalenol( DON) detection method which based on the morphological characteristic of fusarium that produces DON in wheat was preliminary set up. Fusarium conidiophores were the detected target of this method. The spores in the samples were oscillated in water,filtered with 100 mesh filter cloth and the spores in the filtrate were microscopic tested using a microscope. The whole process takes three minutes. The relative standard deviation( RSD) of the wheat positive samples test( n = 6) with different infection levels was 7. 1% ~ 21. 6%. 72 wheat samples collected from main wheat producing areas in China were inspected by this method,and the HPLC method was used as the control. The results showed that fusarium conidiophores were nearly not found in 46 samples,account for 63. 9 % of the total samples,in which DON concentration range were 0 ~ 792. 4 μg / kg which did not exceed the limitation of national standard 1 000μg /kg. The fusarium conidiophores were detected in 26 samples,in which DON were not exceed the limitation in 6 samples,and the growth of fusarium was positively related to the yield of DON. The small batch of wheat can be quickly screened out by this method whether exceeding the DON limitation standard.

关 键 词：小麦DON 筛查 镰刀菌大孢子 

分 类 号：TS211.7[轻工技术与工程—粮食、油脂及植物蛋白工程]