中国和哈萨克斯坦边境阿拉山口口岸蜱中首次检测到巴贝斯虫核酸  

The first detection of Babesia genotype from tick at Alataw Pass, China-Kazakhstan border

作　　者：罗丹[1] 尹小平[2] 王安东[3] 田延河[1] 梁臻[2] 巴特[2] 张江国[2] 

机构地区：[1]新疆石河子大学医学院临床医学系,石河子832000 [2]新疆阿拉山口出入境检验检疫局卫生检疫监管科,阿拉山口833418 [3]新疆石河子大学动物科技学院动物医学系,石河子832000

出　　处：《中华地方病学杂志》2016年第9期633-635,共3页Chinese Journal of Endemiology

摘　　要：目的对中国和哈萨克斯坦（中哈）边境阿拉山口口岸地区蜱虫进行巴贝斯虫核酸检测。方法采用白布旗法和动物体表法在阿拉山口口岸艾比湖湿地采集蜱虫样本。应用聚合酶链式反应（polyreerase chain reaction，PCR）扩增巴贝斯虫18srRNA基因，BLAST分析测序产物，利用Mega6．0构建分子遗传进化树。结果阿拉山口口岸蜱中巴贝斯虫核酸检测阳性率为12．65％（32／253）。经测序，32条巴贝斯虫序列分为ALSK174、ALSK191、ALSK019三组。Blast分析显示：ALSK174与驽巴贝斯虫（EU888904．南非）同源性最高，为99．72％（356／357）；ALSK191与隐藏巴贝斯虫（KP745626，土耳其）同源性最高，为99．72％（350／351）：ALSK019与奥氏巴贝斯虫（KC460321，加拿大）具有95．76％（339／354）同源性。结论首次在中哈边境地区蜱中检测到巴贝斯虫核酸。Objective To investigate the infection state and genotype of Babesia from tick at Alataw port, the China-Kazakhstan border. Methods Drag-flag method and animal body surface method were used to collect ticks at Ebinur Lake wetland, Alataw port. 18s rRNA gene of Babesia was tested by polymerase chain reaction （PCR）, the sequence analysis was conducted with Blast and phylogenetic analysis was conducted with Mega 6.0. Results The positive rate of Babesia gene in ticks was 12.65% （32/253） at Alataw port. By sequencing, 32 sequences were divided in ALSK174, ALSK191, ALSK019 three groups. Analysis of Blast showed that ALSK174 had the highest homology with Babesia cabaUi （EU888904, South Africa）, it was 99.72% （356/357）; ALSK191 had the highest homology with Babesia occultans （KP745626, Turkey）, it was 99.72% （350/351）; ALSKO19 had 95.76% （339/354） homology with Babesia odocoilei （KC460321, Canada）. Conclusion In this study, we have first reported that the Babesia is infected from ticks at Alataw port, the China-Kazakhstan border.

关 键 词：巴贝斯虫 亚洲璃眼蜱 短垫血蜱 聚合酶链式反应 

分 类 号：R38[医药卫生—医学寄生虫学]