谷子CO家族基因的全基因组鉴定与分析  

Genome-wide Identification and Analysis of CO Family Genes in Foxtail Millet

作　　者：穆彩琴[1,2] 屈聪玲 张瑞娟[2,3] 李燕[1,2] 杨致荣[1] 

机构地区：[1]山西农业大学文理学院,山西太谷030801 [2]山西农业大学研究生院,山西太谷030801 [3]山西农业大学生命科学学院,山西太谷030801

出　　处：《山西农业科学》2016年第9期1243-1246,1253,共5页Journal of Shanxi Agricultural Sciences

基　　金：山西省回国留学人员科研资助项目(2015-067);山西省留学回国人员科技活动择优资助项目(2014-11)

摘　　要：抽穗开花是高等植物从营养生长向生殖生长转变的过程,该过程受到众多基因的调控。CONSTANS(CO)是光周期开花途径中的一个关键基因,是生物节律钟基因和开花基因之间的关键枢纽。利用生物信息学的方法,从谷子基因组中鉴定了34个CO基因;与拟南芥CO家族的17个成员进行同源比对,发现Seita.7G007800与Seita.9G020100相似性最高;在此基础上,分析了谷子CO基因的启动子,发现启动子有光响应元件Sp1、脱落酸响应元件ABRE以及赤霉素响应元件P-box等;RNA-Seq转录组测序发现,这些CO基因在叶片中表达量存在显著差异,其中,Seita.4G192300基因表达量最高,而Seita.1G304900,Seita.3G080700和Seita.4G001600这3个基因在叶片中不表达。这些结果不仅为深入研究CO基因与抽穗开花的关系提供依据,而且为后续谷子抽穗开花途径的研究奠定理论基础。Heading and flowering is the transition stage from vegetative to reproductive growth in higher plants, which is regulated by multiple genes. CONSTA NS （CO） is a key gene in photoperiodic flowering pathway and is a key junction between the circadian clock and flowering gene. In this study, 34 CO genes were identified using bioinformatics in foxtail millet. The paper analyzed the relationship between these CO genes and the 17 CO genes in Arabidopsis thaliana, and founded that Seita.7G007800 and Seita.9G020100 had the highest similarity, further analyzed their promoter sequence, and identified light responsive element Spl, ABA response element ABRE, gibberellin-responsive element P-box. RNA-Seq transcriptom sequencing revealed that the expression level of these CO genes was significantly different in leaves. Seita.4G192300 had the highest expression, while Seita.lG304900, Seita.3G080700 and Seita. 4G001600 were not expressed in leaves. These results not only provide a foundation for further study on the relationship between CO genes and flowering, but also contribute to further research on heading and flowering pathways in foxtail millet.

关 键 词：谷子 全基因组 CONSTANS 光周期途径 抽穗 开花 

分 类 号：S515[农业科学—作物学]