循环微小RNA-21水平与稳定性心绞痛患者侧支循环形成的关系  

Relationship between circulating miRNA-21 and collateral circulation formation in stable angina pectoris patients

作　　者：李韶南[1] 刘震[1] 陈平安[1] 李广镰[1] 罗义[1] 雷晓明[1] 

机构地区：[1]广州市第一人民医院心内科,510180

出　　处：《中华老年心脑血管病杂志》2016年第9期920-924,共5页Chinese Journal of Geriatric Heart,Brain and Vessel Diseases

基　　金：广州市科技计划项目(12B113150028)

摘　　要：目的探讨冠状动脉严重狭窄的稳定性心绞痛（SAP）患者循环微小RNA-21（miR-21）与冠状动脉侧支循环（CCC）形成的关系。方法连续入选本院心内科住院的SAP患者130例,患者冠状动脉至少1支血管≥90%狭窄,根据Rentrop分级分为CCC良好组（2~3级）64例和CCC不良组（0~1级）66例,并选同期门诊健康体检者50例为对照组。检测血浆miR-21及血清血管内皮生长因子（VEGF）水平,分析miR-21和VEGF水平与CCC的关系及miR-21与VEGF的相关性。结果 CCC不良组和CCC良好组血浆miR-21及血清VEGF水平均明显高于对照组（P〈0.05,P〈0.01）,且CCC良好组显著高于CCC不良组[（0.86±0.10）2-△△Ct vs（0.74±0.10）2-△△Ct,P〈0.01;（336.7±62.8）ng/L vs（286.2±63.3）ng/L,P〈0.01];血浆miR-21的ROC曲线下面积（AUC）为0.833,血清VEGF的AUC为0.844。CCC良好组血浆miR-21与血清VEGF水平呈正相关（r=0.645,P〈0.05）。CCC不良组血浆miR-21与血清VEGF水平无相关性（r=0.146,P〉0.05）。血浆miR-21和血清VEGF水平是SAP患者CCC良好的独立预测因素（P〈0.05）。结论 SAP患者血浆miR-21及血清VEGF水平与CCC形成良好相关,miR-21通过影响VEGF水平表达,可能是促进患者CCC形成的重要机制之一。Objective To study the relationship between circulating miRNA-21 and coronary collateral circulation（CCC）formation in stable angina pectoris（SAP）patients.Methods One hundred and thirty SAP patients admitted to our hospital from January 2012 to December 2014 were divided into good CCC group（n=64）and poor CCC group（n=66）according to their Rentrop grade with 50 subjects undergoing physical examination served as a control group.Their serum miRNA-21 and VEGF levels were measured.The relationship of circulating miRNA-21 and VEGF levels with CCC formation was analyzed.Results The serum miRNA-21 and VEGF levels were significantly higher in good CCC group than in poor CCC group and control group（0.86±0.102-△△Ct vs 0.74±0.102-△△Ct,P〈0.01;336.7±62.8ng/L vs 286.2±63.3ng/L,P〈0.01）.The AUC under the ROC for serum miRNA-21 and VEGF levels was 0.833 and 0.844 respectively.The serum miRNA-21 level was positively related with the serum VEGF level in good CCC group（r=0.645,P〈0.05）.However,the serum miRNA-21 level was not significantly related with the serum VEGF level in poor CCC group（r=0.146,P〈0.05）.The serum miRNA-21 and VEGF levels were an independent predictor for good CCC in SAP patients（P〈0.05）.Conclusion The serum miRNA-21 and VEGF levels are closely related with the CCC formation,and miRNA-21 promotes the CCC formation by down-regulating the VEGF expression in SAP patients.

关 键 词：冠状动脉狭窄 心绞痛 微RNAS 侧支循环 

分 类 号：R541.4[医药卫生—心血管疾病]