川芎素对结直肠癌Lovo细胞生长的体内外抑制作用及机制  

Inhibitory effect of sodium ferlate on proliferation of human colon cancer Lovo cells in vitro and in vivo

作　　者：金剑[1] 赵庆[1] 

机构地区：[1]上海市第一人民医院宝山分院,上海200940

出　　处：《山东医药》2016年第34期8-10,共3页Shandong Medical Journal

基　　金：上海市卫生和计划生育委员会科研课题(20144Y01116)

摘　　要：目的观察川芎素在体内外对人结直肠癌Lovo细胞生长的抑制作用,并探讨其机制。方法将Lovo细胞分为两组,观察组分别与终浓度为0.125、0.25、0.5、1.0 mg/m L川芎素共培养,对照组不加川芎素。MTT法测定细胞吸光度值,计算细胞活力指数;流式细胞术检测细胞周期和凋亡细胞,计算细胞凋亡率;将14只皮下接种Lovo细胞的裸鼠随机分两组各7只,观察组静脉注射0.5 mg/m L的川芎素50μL,对照组静脉注射等量生理盐水,隔日给药7次,测量给药前后肿瘤体积。采用Western blot法检测Lovo细胞及裸鼠肿瘤组织中的Bcl-2及proCaspase3。结果观察组分别与终浓度0.125、0.25、0.5、1.0 mg/m L川芎素共培养24 h后,其细胞活力指数分别为81.23±6.58、75.69±6.34、62.18±3.29、58.39±2.69,G1期细胞比例减少、S期细胞比例增加(P均<0.05),细胞凋亡率增加(P<0.05);与对照组比较,观察组各时点荷瘤裸鼠肿瘤体积均减小(P均<0.05);观察组Lovo细胞及裸鼠肿瘤组织中的Bcl-2及pro-Caspase3相对表达量较对照组均降低(P均<0.05)。结论川芎素可抑制Bcl-2的表达、激活Caspase-3进而促进凋亡,最终抑制Lovo细胞的体内外生长。Objective To investigate in vitro and in vivo inhibitory effects of sodium ferlate on the proliferation of human colon cancer Lovo cells as well as the relevant mechanism. Methods Lovo cells were divided into two groups： the observation group which was co-cultured with sodium ferlate with the final concentrations of 0. 125,0. 25,0. 5 and 1. 0 mg /m L,and the control group without any sodium ferlate. The optical density of Lovo cells was determined by MTT. The vitality index was calculated. The effects of sodium ferlate on the cell cycle and apoptosis of Lovo cells were analyzed by flow cytometry. Fourteen nude mice bearing Lovo cells were divided into two groups： the observation group which was injected with50 μL sodium ferlate with the concentration of 0. 5 mg / m L,and the control group which was injected by the same amount of normal saline,every other day,for 7 times and the tumor volume was measured before and after medication. The expression of Bcl-2 and pro-Caspase-3 in Lovo cells and tumor tissues was determined by Western blotting. Results The cell vitality indexes were respectively 81. 23 ± 6. 58,75. 69 ± 6. 34,62. 18 ± 3. 29 and 58. 39 ± 2. 69 in the observation group after 24-hour treatment of sodium ferlate with the final concentrations of 0. 125,0. 25,0. 5 and 1. 0 mg / m L. The cell proportion ratio of G1 phase was decreased,and was decreased in the S phase（ all P 0. 05）,meanwhile,the cell apoptosis rate was increased（ P 0. 05）. Compared with the control group,the tumor volume decreased in the tumor-bearing nude mice of the observation group（ all P 0. 05）; and the expression of Bcl-2 and pro-Caspase-3 was decreased in the Lovo cells and tumor tissues of nude mice in the observation group（ all P 0. 05）. Conclusion Sodium ferlate may inhibit the expression of Bcl-2,activate caspase-3 and promote the apoptosis,and thus inhibit the proliferation of Lovo cells.

关 键 词：结直肠癌 川芎素 LOVO细胞 细胞凋亡 B淋巴细胞瘤-2基因 半胱氨酸的天冬氨酸蛋白水解酶 

分 类 号：R966[医药卫生—药理学]