猪霍乱沙门菌菌蜕的制备及免疫保护效力研究  

Preparation and Immune Efficacy of Salmonella choleraesuis Ghost

作　　者：单晓枫[1] 陈龙[1] 李茂辉[1] 康元环[1] 孙武文 张伟[1] 钱爱东[1] 

机构地区：[1]吉林农业大学动物科学技术学院,长春130118

出　　处：《中国兽药杂志》2016年第9期22-25,共4页Chinese Journal of Veterinary Drug

基　　金：吉林省现代农业产业技术体系建设项目(201634)

摘　　要：为制备猪霍乱沙门菌菌蜕并研究其免疫保护效力,克隆噬菌体phi X174裂解基因E,与p BV220连接,构建温控溶菌表达载体,将该载体转入猪霍乱沙门菌TTB1中,制备菌蜕并对其裂解率、安全性以及对小鼠的免疫保护效力进行了研究。结果显示:成功克隆了裂解基因E、片段大小274 bp,构建了温控溶菌质粒载体p BVE,制备了猪霍乱沙门菌菌蜕,其裂解率最高为99.46%、经冻干灭活残余活菌,安全性试验检测证实其安全后,对小鼠进行了免疫保护试验,其保护率为70%、与弗氏佐剂灭活苗保护率相当、优于甲醛灭活苗。研究为猪霍乱沙门菌感染的防治奠定了基础。In order to develop a Salmonella choleraesuis bacterial ghost vaccine and investigate its protective immunity. This study, we cloned the lysis expression plasmid pBV220 vector, and th choleraesuis ghost and studied its lytic effic about 274 bp was clone, the temperature - gene E from phage phiX174 and inserted into an temperature - induced len wastransformed into Salmonella TFB1. We created the Salmonella iency and protective immunity. The results showed that the lysisgene E induced lytic plasmid pBVE and Salmonella choleraesuis bacterial ghost were created. The lysis rate can be 99.46% and no life bacteria survived after freeze drying, The results of protective immunity showed that Salmonella ghost group providethe protection rate of 70%, The protection rate ofplasmid - type Salmonella adjuvant, which was better ghost vaccine than that of strain was comparable that of Salmonella inactivated vaccine with the inactivated vaccine. The research provides a foundation for the prevention and treatment of diseases caused by Salmonella choleraesuis

关 键 词：猪霍乱沙门菌 菌蜕 免疫保护效力 裂解基因E 裂解效率 

分 类 号：S852.61[农业科学—基础兽医学]