低温处理下东农冬麦1号小麦根组织EXPA基因的表达分析  

Expression Analysis of EXPA Gene in Root Tissues of Winter Wheat Dongnongdongmai 1 under Low Temperature Treatment

作　　者：李飞[1] 王晓磊[2] 徐永清[1] 张俊峰[1] 董佳敏[1] 苗宇[1] 冯旭[1] 李凤兰[1] 胡宝忠[1,2] 

机构地区：[1]东北农业大学生命科学学院,黑龙江哈尔滨150030 [2]哈尔滨学院,黑龙江哈尔滨150086

出　　处：《麦类作物学报》2016年第9期1159-1166,共8页Journal of Triticeae Crops

基　　金：国家基础科学人才培养基金项目(J1210069);中俄国际合作项目(2013DFR30270)

摘　　要：寒地冬小麦膨胀素(Expansin)基因的表达特性同根系建成密切相关,而形成发达的根系是高寒地区冬小麦成功越冬返青的关键。为明确低温对高寒地区冬小麦膨胀素基因表达特性的影响,进而提高冬小麦对寒冷的适应性,以高抗寒冬小麦东农冬麦1号为试验材料,以正常冬小麦济麦22号为对照材料,运用实时荧光定量PCR技术对经不同低温处理的小麦幼苗根组织中编码α-expansin(EXPA)的基因TaEXPA5、TaEXPA6和TaEXPA7的表达特性进行分析。结果表明,在4℃、-10℃和-20℃低温处理下,高抗寒冬小麦东农冬麦1号中TaEXPA5、TaEXPA6和TaEXPA7基因的相对表达量与对照相比呈现显著性上升,其中,4℃处理条件下,TaEXPA6和TaEXPA7基因的相对表达量变化幅度较明显,-10℃处理条件下,TaEXPA5基因的相对表达量变化幅度较明显,-20℃处理条件下,TaEXPA5和TaEXPA7基因的相对表达量变化幅度较明显。说明低温影响小麦根组织中TaEXPA5、TaEXPA6和TaEXPA7基因的表达,三个基因与抗御低温胁迫具有相关性,并且各基因表达存在一定的协作性。The expression characteristics of expansin genes in winter wheat in cold region are closely as- sociated with the establishment of root system. Thus, forming developed root system is crucial for winter wheat to overwinter and return green successfully in cold region. In order to investigate the effect of low temperature on the expression characteristics of expansin genes in winter wheat and enhance its adaptation in chilling condition,in this study, the expression characteristics of seedling root tissues TaEXPA5, TaEXPA6 and TaEXPA7 genes were analyzed in different low temperature by quantitative real-time PCR with the cold-resistance variety Dongnongdongmai 1 as experiment material and cold-sensitive variety Jimai 22 as control. The results showed that compared with control sample, the expression of TaEXPA5, TaEXPA6 and TaEXPA7 genes in Dongnongdongmai 1 was significantly higher under 4 ℃, -10 ℃ and -20 ℃ ,respectively. The relative expression of TaEXPA6 and TaEX-PAT genes changed distinctly under 4 ℃. Similarly, under the treatment o! -- I0 - - tne vartauon ul TaEXPA5 expression was definitely large. At the meanwhile,the relative expression of TaEXPA5 and TaEXPA7 genes changed significantly under the treatment of -20 ℃. It indicates that low temperature has effect on the expression of TaEXPA5, TaEXPA6 and TaEXPA7 genes in root tissues of winter wheat. These three genes are correlated to resist the low temperature stress and there exists certain cooperation among the expression of the three genes.

关 键 词：低温 高抗寒冬小麦 EXPA 实时定量PCR 

分 类 号：S512.1[农业科学—作物学] S330