比色法测定硫酸阿米卡星滴鼻剂含量  

Determinition of amikacin sulfate nasal drops by colorimetric

作　　者：钟志东[1] 李之琳[1] 

机构地区：[1]天津市儿童医院,天津300074

出　　处：《中国医院药学杂志》2002年第8期479-480,共2页Chinese Journal of Hospital Pharmacy

摘　　要：目的 :建立一种简便、可靠的检测方法用于硫酸阿米卡星滴鼻液含量测定。方法 :在一定 pH值和加热条件下 ,茚三酮与阿米卡星发生络合反应生成紫色络合物 ,采用比色法进行测定。测定波长为 5 6 7nm处。结果 :阿米卡星在 12～ 2 2 μg·ml-1浓度范围内与吸收度呈良好的线性关系 ,r =0 .9995 (n =6 ) ,平均回收率为 99.10 % ,RSD =0 .8% (n =5 )。结论 :该方法简便 ,快速 ,结果准确 。OBJECTIVE To establish a simple rapid method for the determination of amikacin sulfate nasal drops.METHODS Amikacin can react with ninhydrine to form a purple stable compound which can be detected at 567 nm .in appropriate condition of heat and pH.RESULTS The calibration curve was linear in the range of 12～22 μg·ml -1 , r= 0.9995 ( n =6).The average recovery was 99.10% .RSD= 0.8% ( n =5).CONCLUSIONS The method is simple,rapid,accurate and can be used for quantitative control of the prepartions.

关 键 词：硫酸阿米卡星滴鼻剂 含量测定 比色法 医院制剂 质量控制 

分 类 号：R987[医药卫生—药品] R927.2[医药卫生—药学]