凉血通瘀方治疗急性脑出血大鼠的疗效及机理研究  

Experimental Research of Effect and Mechanism of Liangxue Tongyu Recipe on Acute Cerebral Hemorrhage in Rats

作　　者：陈叶香[1,2] 李国春[1] 许立[2] 尹莲[2] 王均琴[1] 秦蕾[1] 刘德麟[3] 

机构地区：[1]南京中医药大学医学与生命科学学院,江苏南京210023 [2]南京中医药大学药学院,江苏南京210023 [3]中国中医科学院中医基础理论研究所,北京100700

出　　处：《广州中医药大学学报》2016年第5期683-687,共5页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：国家自然科学基金面上项目(编号:81373512);江苏省自然科学基金面上项目(编号:BK20161575)

摘　　要：【目的】观察凉血通瘀方对急性脑出血大鼠的干预作用,评价凉血通瘀方不同提取物对急性脑出血的疗效及其作用机理。【方法】12只SD大鼠等比例随机分为假手术组、模型组、水提组和醇提组。除假手术组大鼠左侧尾状核注入2μL的生理盐水外,其他组的大鼠左侧尾状核内均注入2μL(0.25 U/μL)Ⅶ型胶原酶复制大鼠急性脑出血模型,给药组每天灌胃给予不同提取方法的凉血通瘀方。观察指标包括大鼠大脑大体形态,脑组织病理检查,大鼠苏醒时间、肛温、行为学评分以及血清特异性烯醇化酶(NSE)测定。【结果】术后除假手术组外,各组大鼠均具有不同程度的右侧肢体偏瘫症状,且在第3天最为严重,凉血通瘀方醇提组和水提组对脑出血大鼠神经功能学评分具有改善作用,能加速其苏醒、降低其肛温及血清NSE水平。【结论】凉血通瘀方水提液和醇提液均对Ⅶ型胶原酶诱导的急性脑出血具有一定的改善作用。Objective To observe the effect and mechanism of extracts of Liangxue Tongyu Recipe（LTR）on acute cerebral hemorrhage rats. Methods Twelve SD rats were randomly divided into sham operation group,model group, LTR water-extract group and LTR alcohol-extract group. Rats of sham operation group were injected with 2 μL of normal saline into the left caudate nucleus,and rats of the other groups were injected with 2μL（0.25 U/ μL） of type Ⅶ collagenase into the left caudate nucleus to induce the model of acute cerebral hemorrhage. And then the rats of LTR groups were given intra-gastric gavage of LTR water-extract or LTR alcohol-extract. At the end of the experiment,we observed the macroscopic features of rat brain,pathological changes in rat brain under microscope after HE staining, awake time, rectal temperature, neurobehavioral scores and serum-specific enolase（NSE） level. Results Rats except for the sham operation group had various degrees of right hemiplegia symptoms,and the symptoms became severest on the third day of experiment. LTR alcohol-extract group and water-extract group had an effect on improving neurobehavioral scores, shortening awake time, and decreasing rectal temperature and serum NSE level of model rats. Conclusion LTR alcohol-extract and water-extract can relieve acute cerebral hemorrhage induced by type Ⅶ collagenase.

关 键 词：凉血通瘀方/药理学 急性脑出血 特异性烯醇化酶 脑/病理学 疾病模型 动物 大鼠 

分 类 号：R285.5[医药卫生—中药学]