燕窝提取物抗H5N1禽流感病毒的作用及机理研究  

Effect and Mechanism of Extracts of Nidus Collocaliae on Resisting Avian Influenza A H5N1 Virus

作　　者：林洁茹[1] 张莹[1] 戴卫平[1] 刘叔文[2] 李耿[1] 周华[3] 赖小平[1] 

机构地区：[1]广州中医药大学中药学院,广东广州510006 [2]南方医科大学药学院,广东广州510515 [3]澳门科技大学

出　　处：《广州中医药大学学报》2016年第5期710-715,共6页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金："十二.五"国际科技合作项目(编号:2014DFH30010);广东省科技计划项目(编号:2013B090800052;2013B020224005);广东普通高校国家级重大培育项目(编号:E1-KFD015151K02)

摘　　要：【目的】观察燕窝提取物体外抑制H5N1禽流感病毒的作用。【方法】制备燕窝水提物、燕窝水提物人工胃液消化物、燕窝水提物人工肠液消化物。通过体外293T细胞转染方法,应用荧光素酶检测试剂盒检测3种燕窝提取物对H5N1禽流感假病毒、VSV-G假病毒活性的影响;观察禽流感病毒H5、H7、H9 3种亚型抗原凝血反应及3种燕窝提取物对其抑制作用;应用神经氨酸酶抑制剂筛选试剂盒检测3种燕窝提取物对N1型神经氨酸酶(NA)活性的影响。【结果】3种燕窝提取物对H5N1禽流感假病毒活性均有抑制作用,抑制作用的强度随燕窝提取物浓度增高而增加,其中人工肠液消化物作用最强,水提取物最弱,但对VSV-G假病毒活性均无显著抑制作用。H5、H7型抗原凝血价为1∶128,H9型抗原凝血价为1∶256,3种燕窝提取物在一定浓度条件下对H5、H7、H9抗原血凝反应均有抑制作用,但对NA均没有抑制作用。【结论】燕窝提取物抗病毒的作用可能是通过抑制血凝素的活性而实现的。Objective To observe the inhibitory effect of the extracts of Nidus Collocaliae on avian influenza A H5N1 virus in vitro. Methods Nidus Collocaliae water extract,artificial gastric juice digestion products of Nidus Collocaliae water extract,and artifitial intestinal juice digestion products of Nidus Collocaliae water extract were prepared for the experimental study. 293 T cells transfection in vitro was carried out. The effects of 3 kinds of Nidus Collocaliae extracts on H5N1 pseudovirus and VSV-G pseudovirus were determined by luciferase detection kit. Blood clotting response to erythrocyte hemagglutinin subtypes H5, H7, H9 antigens and the inhibitory effects of 3 kinds of Nidus Collocaliae extracts were observed. The effects of 3 kinds of Nidus Collocaliae extracts on neuraminidase activity were determined by neuraminidase inhibitor screen kit. Results The 3 kinds of Nidus Collocaliae extracts had inhibitory effects on H5N1 avian influenza pseudovirus,the effects being enhanced with the increase of the concentrations of Nidus Collocaliae extracts. Of the 3 extracts, artificial intestinal digestion products had the strongest inhibitory effect, while Nidus Collocaliae water extract had the weakest effect.However,Nidus Collocaliae extracts had no obvious effect on VSV-G pseudovirus. The concentration of H5,H7 antigen for positive blood clotting response was 1 ∶ 128, and that of H9 antigen was 1 ∶ 256. The 3 kinds of Nidus Collocaliae extracts at certain concentrations could inhibit blood clotting response to H5, H7, H9 antigen, but had no obvious effect on neuraminidase. Conclusion The anti-H5N1 virus effect of Nidus Collocaliae extracts has been achieved probably through resisting hemagglutinin.

关 键 词：燕窝提取物/药理学 H5N1禽流感病毒 细胞转染 凝血反应 

分 类 号：R285.5[医药卫生—中药学]