阿托伐他汀对脂多糖诱导人主动脉内皮细胞细胞间黏附分子-1表达的影响  

Effect of Atorvastatin on the Expression of Intercellular Adhesion Molecule-1 in Cultured Human Aortic Endothelial Cells Induced by Lipopolysaccharide

作　　者：嵇云鹏 张彦燕[1] 杨红[1,3] 石廷雨 徐旖旎[1] 陶玲[1] 沈祥春[1,3] 

机构地区：[1]贵州医科大学天然药物资源优效利用重点实验室,贵州贵阳550025 [2]贵州省人民医院药剂科,贵州贵阳550002 [3]贵州医科大学中药药理教研室,贵州贵阳550025

出　　处：《贵阳医学院学报》2016年第8期882-886,共5页Journal of Guiyang Medical College

基　　金：国家自然科学基金资助项目(81360650;81560811);贵州省留学人员科技活动项目[黔人资合(2013)02号];贵州省高层次创新型人才[黔科合人才(2015)4029号];贵阳市现代药业计划[筑科合同(2011204)17号];贵州省中药现代化专项[黔科合中药字(2012)5051号];贵州省高等学校创新团队[黔教合人才团队(2014)31号];贵州省创新团队[黔科合人才团队(2015)4025号]

摘　　要：目的:观察阿托伐他汀(Atv.)对脂多糖(LPS)诱导人主动脉内皮细胞(HAECs)细胞间黏附分子-1(ICAM-1)表达的影响,并探讨其分子机制。方法:用1 mg/L LPS复制HAECs损伤模型,通过MTT和乳酸脱氢酶(LDH)外漏率实验观察5μmol/L Atv.对LPS诱导HAECs损伤的保护作用,采用RT-PCR法检测Atv.对ICAM-1 mRNA表达的影响,Western blot检测Atv.对ICAM-1、IκBα及NF-κB蛋白表达水平的影响。结果:与LPS组比较,Atv.能有效抑制LPS诱导的HAECs损伤,细胞活力增加(P<0.01),LDH外漏率减少(P<0.01),Atv.可抑制LPS诱导的ICAM-1 mRNA(P<0.05)和蛋白表达上调(P<0.01),还可抑制LPS诱导的IκBα蛋白表达水平的降低和phospho-NF-κB p65蛋白表达水平的升高(P<0.01)。结论:阿托伐他汀对LPS诱导HAECs损伤具有保护作用,并有效抑制ICAM-1的表达,该作用与抑制IκBα降解及NF-κB p65磷酸化有关。Objective： To observe the effect and mechanism of atorvastatin on the expression of ICAM-1 in lipopolysaccharide （LPS）-induced injury of cultured human aortic endothelial cells （HAECs）. Methods： Cultured HAECs were treated with LPS alone （1 nag/L） or in the presence of atorvastatin （Atv. ,5 μmol/L）. MTF and LDH leakage ratio were used to analyze the protective effect of atorvastatin against LPS. The RT-PCR was used to determine the mRNA expression of ICAM-1. The western blot was adopted to detect the protein expression of ICAM-1, IκBα, NF-κB in HAECs injury induced by LPS. Results： Compared with LPS group, atorvastatin could effectively inhibit LPS-in- duced injury by increasing cell activity（ P 〈 0.01 ）, decreasing LDH releasing ratio（ P 〈 0.01 ）, inhib- iting the up-regulating mRNA（P 〈 0.05） and protein （P 〈 0.01 ） expression of ICAM-I, inhibiting the decreasing of phospho-NF-κB p65 protein expression （P 〈 0.01 ） and the increasing of IκBα pro-tein expression （P 〈0. O1 ）. Conclusion： Atorvastatin has a protective effect on LPS induced HAECs injury, and can effectively inhibit the expression of ICAM-1, which is related to the inhibition of IκBα degradation and NF-κB p65 phosphorylation.

关 键 词：阿托伐他汀 人主动脉内皮细胞 细胞间黏附分子-1 核因子-KB 

分 类 号：R96[医药卫生—药理学]