右美托咪定对单肺通气大鼠术后早期空间记忆功能的影响  

Dexmedetomidine Improves the Early Spatial Memory Function in Rats Undergoing One-lung Ventilation

作　　者：鲍冲[1] 金哲[1] 柯剑娟[1] 陈凯[1] 王焱林[1] 

机构地区：[1]武汉大学中南医院,湖北武汉430071

出　　处：《贵阳医学院学报》2016年第8期891-895,共5页Journal of Guiyang Medical College

基　　金：国家自然科学基金项目(81471858)

摘　　要：目的：探讨右美托咪定对单肺通气大鼠术后早期空间记忆能力的影响。方法：选择雄性SD大鼠72只，月龄10～11月，随机分为双肺通气组（TLV组）、单肺通气组（OLV组）和右美托咪定组（DEX组）；DEX组于诱导前30min腹腔注射盐酸右美托咪定25μg/kg，TLV组与OLV组给予等量生理盐水腹腔注射；各组大鼠采用异氟烷诱导并维持麻醉，经口气管插管，TLV组全程双肺通气，OLV组和DEX组双肺通气5min后采用插入过深法行单肺通气，90min后恢复双肺通气30min；每组各取6只大鼠，行右颈内静脉、左侧颈总动脉逆血流置管，以开始单肺通气0、30、60、90和120min时（分别记作T0、T1、T2、T3、T4）采集右颈内静脉和左侧颈总动脉血行血气分析，计算脑氧摄取率（CERO2）；各组剩余18只大鼠，于术前5d开始每天行Morris水迷宫定向航行训练，并于术后第1、3、7天时行空间探索实验，记录各组大鼠逃避潜伏期、目标象限停留时间和穿台次数。结果：随着术后通气时间延长，各组CERO2逐渐上升（P〈0．01），OLV组在T1至T4时点CERO2明显高于TLV组（P〈0．01），恢复双肺通气后，仍高于TLV组；DEX组在T1至T4时点CERO：均明显低于OLV组（P〈0．05或P〈0．01）；各组大鼠随着术前训练天数的增加，逃避潜伏期逐渐缩短（P〈0．01）；OLV组在术后第1、3、7天，逃避潜伏期均较TLV组明显延长（P〈0．01），目标象限停留时间缩短（P〈0．01），穿台次数减少（P〈0．01）；DEX组在术后第1、3、7天逃避潜伏期均短于OLV组，且目标象限停留时间增加，穿台次数增加（P〈0．05）。结论：右美托咪定可以改善单肺通气大鼠术后早期空间记忆功能，其机制可能与降低脑组织氧耗有关。Objective： To investigate the effect of Dexmedetomidine on the early spatial memory func- tion in rats undergoing one-lung ventilation surgery. Methods： 72 male sprague-dawley rats were ran- domly divided into 3 groups with 24 of each ： group of two-lung ventilation （ group TLV）, group of one- lung ventilation （group OLV ） and group of one-lung ventilation accompanied with the injection of Dexmedetomidine（ group DEX）. Group DEX received Dexmedetomidine by intraperitoneal injection with a dose of 25 μg,/kg in 30 minutes before induction, while an equal volume of saline was given to group TLV and group OLV at the same time. All three groups receive isoflurane anesthesia （with a flow of 1.5% ） , two-lung ventilation were given for 5min after intubation for each group followed by one-lung ventilation for 90min and lung inflate for 30min for group OLV and group DEX while only two-lung ventilation were given for group TLV. 6 rats of each group were chosen randomly to capture blood samples from left carotid artery and right internal jugular vein at the time of the beginning（T0）, 30 rain（ T1 ）, 60 rain（ T2 ） ,90 min （ T3 ） of one-lung ventilation and 30 rain after lung inflate （ T4 ） for blood gas analysis to calculate CEROz. The remaining rats in each group were divided randomly into 3 subgroups with 6 of each. The spatial memory function was evaluated by Morris Water Maze （MWM）. The place navigation test was taken for five consecutive day before ventilation. The 3 subgroups had the spatial probe test respectively on 1, 3 or 7 post-operative day （post-1d, post-3d, post-7d） for detec- ting escape latency（ EL）, percentage of time spend in target quadrant and times of cross over the plat- form. Results： CEROz in every groups increased gradually while ventilation. Compared with group TLV, CERO2 increased significantly at T1 ,T2 ,T3 ,T4 in group OLV（P 〈0.01 ） . Compared with group OLV, CERO2 were lower in group DEX at T1 ,T2 ,T3 ,T4 （P 〈 0.05 or P 〈 0.01 �

关 键 词：右美托咪定 单肺通气 脑氧摄取率 认知功能障碍 

分 类 号：R453.9[医药卫生—治疗学] R35-33[医药卫生—临床医学]