成人腹股沟疝发病机制的基因组学变异研究  

Observations of Genes Variantions in Primary Adult Ingunial Hernias

作　　者：聂鑫[1] 宋致成[1] 陈渊文[1] 顾岩[1] 

机构地区：[1]上海交通大学医学院附属第九人民医院普外科,上海交通大学疝与腹壁外科疾病诊治中心,上海200011

出　　处：《医学临床研究》2016年第8期1457-1460,共4页Journal of Clinical Research

基　　金：国家自然科学基金（81270444、81470792）

摘　　要：[目的]探讨成人原发性腹股沟疝基因组学的相关变异。[方法]利用基因芯片技术对原发性腹股沟疝患者的腹横筋膜与正常腹横筋膜进行检测比较，筛选表达差异的相关基因，挑选差异程度最高的目标基因进行RT-qRCR检验。[结果]基因芯片筛选两组标本得到1189个差异表达基因，其中包含877个表达上调基因和312个表迭下调基因，其中肌球蛋白调节轻链9（MYL9）是差异最显著的上调基因，而肌球蛋白调节重链1（MYH1）是差异最显著的下调基因，对MYL9和MYH1进行RT-qRCR检验，结果与芯片结果一致。[结论]成人腹股沟疝的发病可能与相关基因差异性表达相关，其中MYL9及MYH1可能起着重要作用。[ObjectivelTo explore the genomic variations in adult primary inguinal hernia. [Methods]Utili- zing gene chips to scan transversalis fascia of patient with ingunial hernia, the results were compared with cor~ responding genomes of contro[ patients. The genes with the highest different regulation degrees were chosen to be further examined using the RT-qPCR method. [Results]From the array analysis performed on the two groups, 1189 mRNAs were found to be expressed differently between the groups-877 mRNAs were upregulat- ed and 312 mRNAs were downregulated. Of the mRNAs, MYL9 showed the highest degree of upregulation and MYH1 showed the higheset degree of downregulation. We chose the MYL9 and MYH1 as target genes for further examination with RT-qPCR. The consistency between the RT-qPCR results and microarray data was shown. [Conclusion]The occurrence and development of inguinal hernia may be associated with a multitude of genetic variations, hut MYL9 and MYH1 may play the most significant roles.

关 键 词：疝 腹股沟/病理生理学 疝 腹股沟/遗传学 基因组学 变异(遗传学) 肌球蛋白类 

分 类 号：R656.21[医药卫生—外科学]