电针“曲池”“足三里”穴对缺血再灌注损伤大鼠纹状体DARPP-32磷酸化的影响  

Effects of Electroacupuncture at Quchi(LI11) and Zusanli(ST36) on DARPP-32 Phosphorylation in Rat Striatum after Cerebral Ischmeia-reperfusion

作　　者：林云娇 王露露[1,2,3] 吴洁[1,2,3] 黄佳[1,2,3] 宋长明[1,2,3] 林冰冰[1,2,3] 柳维林[1,2,3] 陶静[1,2,3] 陈立典[1,2,3,4] 

机构地区：[1]福建中医药大学康复医学院,福建福州350122 [2]福建省康复技术重点实验室,福建福州350122 [3]福建康复产业研究院,福建福州350122 [4]国家中医药管理局中医康复研究中心,福建福州350122

出　　处：《中国中医急症》2016年第9期1645-1648,1739,共5页Journal of Emergency in Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81273835;81373778);福建省卫生厅青年科研课题(2014-1-70)

摘　　要：目的观察电针"曲池""足三里"穴对脑缺血再灌注损伤局灶性大脑中动脉闭塞模型(MCAO)大鼠神经行为学的变化及多巴胺和环磷酸腺苷调节的蛋白(DARPP-32)磷酸化的影响。方法将25只雄性SD大鼠随机分为假手术组(6只)、手术组(19只)。手术组采用Longa线拴法制备左侧左侧大脑中动脉栓塞(MCAO)大鼠模型,将造模后符合纳入标准的12只大鼠随机分为模型组、电针组,各6只。电针组取"曲池"和"足三里"穴,电针干预14 d,假手术组和模型组在同等条件下抓取及固定,不予任何干预。通过神经功能评分判断大鼠的神经功能缺损情况;小动物磁共振仪(MRI)扫描观察脑梗死体积,Western blot检测纹状体DARPP-32、p-Thr75-DARPP-32的表达情况。结果与模型组相比,电针组大鼠神经功能评分降低(P<0.05),脑梗死体积减少(P<0.05),纹状体p-DARPP-32-Thr75/DARPP-32比值增高(P<0.05)。结论电针"曲池"和"足三里"穴可改善MCAO大鼠神经功能缺损症状,其机制可能与促进缺血纹状体的DARPP-32在Thr75位点的磷酸化有关。Objective： To observe the effect of electro-acupuncture at Quchi （LI11 ） and Zusanli （ST36） on the change of neurological deficit,and the phosphorylation of dopamine and cyclic adenosin 3＇ ,5＇-monophosphate regulated phosphoprotein （DARPP-32）in rat after cerebral ischemia reperfusion injury. Methods： 25 male SD rats were randomly divided into the sham operation group （n = 6） and the operation group （n = 19）. The operation group were under the operation of the left middle cerebral artery occlusion（MCAO） by the modified Longa＇ meth- ods ,and 12 qualified rats were randomly divided into the model group （n = 6） and the electro-acupuncture group （n = 6）. The electro-acupuncture group received electro-acupuncture at Quchi （LI11 ） and Zusanli（ST36） for 14 days. The neurological deficit was assessed;the cerebral infarct volume was scanned with animal magnetic reso- nance imaging （MRI） machine~ The protein expression of DARPP-32 and p-Thr75-DARPP-32 were detected by Western blotting technique. Results： Compared with the model group,the neurological deficit score in the elec- tro-acupuncture group was improved （P〈 0.05）. The size of infarct volume was significantly reduced （P〈 0.05）. The radio of p-DARPP-32-Thr75/DARPP-32 in corpus striatum in electro-acupuncture group was higher than that in the model group （P〈 0.05 ）. Conclusion： The electro-acupuncture at Quchi （LI 11 ） and Zusanli （ST36） can promote the recovery of neurologic deficit symptom,which may be associated with promoting the phosphorylation of DARPP-32 on Thr75.

关 键 词：脑缺血 电针 DARPP-32 p-DARPP-32-Thr75 

分 类 号：R245.97[医药卫生—针灸推拿学]