续断水提液对小鼠胫骨骨折愈合影响的实验研究  

Experimental Study on Promoting Effect of Radix Dipsaci Extract on Tibial Fracture Healing in Mice under Micro-CT

作　　者：武春明 袁慧敏[1,2] 杨迪[3] 刘魏[1,2] 毛矛[1,2] 邹斌杰 苏彪[1,2] 胡伟龙[1,2] 刘晋闽[1,2] 

机构地区：[1]浙江中医药大学,浙江杭州310053 [2]浙江省中医院,浙江杭州310006 [3]浙江省人民医院,浙江杭州310014

出　　处：《中国中医急症》2016年第9期1682-1685,1689,共5页Journal of Emergency in Traditional Chinese Medicine

基　　金：浙江省中医药科技计划一般项目(2014ZB008)

摘　　要：目的观察续断水提液对小鼠胫骨横行骨折愈合过程的影响。方法选用12周龄C57小鼠70只,无菌条件下实施胫骨干横形骨折髓内钉固定术,胫骨X线正侧位片剔除不合格小鼠,余下随机分为模型组、治疗组,每组再随机分为5个不同时间点。治疗组予续断水提液灌胃,模型组给予同体积生理盐水,分别于造模后第4天、7天、10天、14天、21天取样,固定后X线观察骨折愈合大体情况,Micro-CT测量ROI总体积(TV)、骨痂总体积(BV)、骨体积分数(BV/TV)和骨小梁相关参数,同时通过组织形态学HE染色评估续断水提液对小鼠骨折愈合过程的形态学影响。结果 X线显示两组在骨折愈合早期(4、7、10 d)两组骨折线多较清晰,而在骨折愈合中后期(14、21 d)治疗组骨折线模糊程度高于模型组(P<0.05);Micro-CT显示两组间BV、BV/TV、Tb.Pf和SMI在术后14 d、21 d差异均具有统计学意义(P<0.05),而TS两组间在各时间点差异均无统计学意义(均P>0.05);术后7 d和10 d时,骨痂多为软组织,14 d和21 d则逐渐向骨性转化。在术后14 d和21 d两个时间点,治疗组骨痂骨量明显多于模型组,而且治疗组骨痂的形状也更为规则,与皮质骨贴合更为紧密。结论依据X线、Micro-CT及组织形态学HE染色发现续断水提液可促进小鼠胫骨横行骨折的愈合,且其作用时间点为小鼠骨折愈合中后期。Objective： To explore the promoting effect of Radix Dipsaci extract on tibial fracture healing in mice. Methods： 12-week-old C57BL/6 mouse were chosen for the experiment,and made a unilateral open tibiae transverse fracture with intramedullary needle fixation model. After the operation ,They were divided into 2 groups for 5 time points randomly. The treatment group and the model group were given Teasel root extract and saline re- spectively. Thesamples were taken on 4th,7th,10th, 14th and 21st day. 3 days later,X-ray was used to observe the general situation of fracture healing,and the characteristics of bone volume of ROI （TV） ,volume of callus（BV）, bone volume fraction （BV/TV） and Trabeeular related indexes were detected with Micro-CT. At the same time, histological staining （HE） was used to assess the morphological effect of Radix Dipsaci extrac on fracture healing process of mice. Results： X-ray showed that in the early fracture healing （4th, 7th, 10th day）, most of fracture lines of two groups were clear,but in the late stage of fracture healing （14th,21st day）,the fuzzy degree of the treatment group was higher than that of the model group. Micro-CT showed that there were statistical differences in BV, BV/ TV,Tb.Pf,and SMI between the treatment group and the model group on 14~ day and 21st day after the operation （P 〈 0.05）. TS showed no statistical differences among all time points. Morphological HE staining showed that 10th, 14th and 21st day after operation,callus quantity,shape and direction of the treatment group were better than those of the model group. Conclusion： Radix Dipsaci extract has a promoting effect on tibial fracture healing in mice and the promoting effect focuses on the middle and late period.

关 键 词：骨折 MICRO-CT 续断 骨折 

分 类 号：R285.5[医药卫生—中药学]