抗原原罪对 H5N1流感疫苗免疫效果的影响  

Effects of original antigenic sin on the immune response to inactivated influenza A virus subtype ;H5N1 vaccine

作　　者：谢震渊[1] 彭纳[2] 张亚楠[2] 毛树宝 陈则[3] 常海艳[2] 

机构地区：[1]上海市徐汇区斜土街道社区卫生服务中心,200032 [2]湖南师范大学生命科学学院,长沙410081 [3]上海生物制品研究所有限责任公司,200052

出　　处：《中华微生物学和免疫学杂志》2016年第8期566-571,共6页Chinese Journal of Microbiology and Immunology

基　　金：湖南省科技计划项目（2013SK3131）;湖南省高校创新平台开放基金（15K083） 志谢：感谢湖南省生物发育工程及新产品研发协同创新中心对本研究的帮助.

摘　　要：目的：探讨针对H1 N1流感病毒产生的免疫效应，是否由于抗原原罪的存在，而对H5N1流感疫苗的免疫应答产生影响。方法以BALB/c小鼠为动物模型，预先用流感病毒A/PR8感染小鼠或用A/PR8的流感病毒灭活疫苗免疫小鼠，使得小鼠产生针对A/PR8流感病毒的免疫应答，再分别用0.01μg、0.1μg和1μg的H5N1流感病毒灭活疫苗免疫一次，检测小鼠免疫后第1、2、3周产生的IgG抗体和中和抗体，并在小鼠免疫H5N1疫苗后的第4周，用致死性的H5N1流感病毒攻击小鼠，观察小鼠的存活和体重丢失以及小鼠肺部病毒滴度判断抗原原罪对H5 N1疫苗的免疫效果的影响。结果免疫H5 N1流感病毒疫苗后，产生的抗体滴度和对流感病毒感染的保护效应，没有因为预先存在的针对A/PR8的抗体而减弱。结论在本实验中表明抗原原罪没有影响H5N1疫苗的免疫效应。Objective To investigate the effects of original antigenic sin caused by previous expo-sure to influenza A virus subtype H1N1 on the immune response to inactivated H5N1 vaccine. Methods In this study, the BALB/c mice were first infected with A/PR8 （H1N1） virus or immunized with inactivated vaccine to induce immune responses against the A/PR8 virus. Then they were injected once with inactivated H5N1 vaccine at dosages of 0. 01μg, 0. 1μg and 1μg, respectively. The levels of IgG and neutralizing an-tibodies in serum samples were detected after immunization. Four weeks after immunization, the mice were challenged with a lethal dose of H5N1 virus. Some indicators including the survival rate, body weight loss and residue virus titer in lung were recorded for further evaluation. Results The pre-existing anti-A/PR8 antibodies in mice didn′t alleviate the immune responses to inactivated H5N1 vaccine. Conclusion This study indicates that the original antigenic sin associated with the previous exposure to A/PR8 virus has no significant effect on the immune efficacy of H5N1 vaccine.

关 键 词：流感疫苗 抗原原罪 H5N1流感病毒 

分 类 号：R392-33[医药卫生—免疫学]