河南睢县3岁以下儿童携带屎肠球菌耐药性及多位点序列分型研究  

Antibiotic resistance and multilocus sequence typing of Enterococcus faecium strains isolated from ;children under 3 years old in Sui county, Henan province

作　　者：胡守奎[1] 周娟[1] 胡园园[1] 熊衍文[1] 金东[1] 

机构地区：[1]中国疾病预防控制中心传染病所,传染病预防控制国家重点实验室,北京102206

出　　处：《中华微生物学和免疫学杂志》2016年第8期588-592,共5页Chinese Journal of Microbiology and Immunology

基　　金：国家自然科学基金重大课题（81290340,81290345）

摘　　要：目的：了解河南睢县3岁以下儿童携带屎肠球菌耐药性和携带毒力基因情况,并对分离菌株进行多位点序列分型研究。方法应用K-B纸片法检测屎肠球菌对15种常见抗生素的耐药性；PCR方法检测屎肠球菌携带毒力基因的情况；多位点序列分型（ multilocus sequence typing, MLST）方法对分离菌株进行分型。结果从120份河南睢县3岁以下儿童粪便标本中分离到47株屎肠球菌。在47株屎肠球菌中耐药菌株的比例为95.7%,分离菌株对大环内酯类、四环素以及利福平等多种抗生素广泛耐药,对临床常用于屎肠球菌感染治疗的β-内酰胺类以及氨基糖苷类抗生素耐药率亦高。未检测到对万古霉素、利奈唑胺、氯霉素和呋喃妥因耐药的菌株。3种以上抗生素耐药菌株都属于一个克隆群,其中包括12株携带透明质酸酶（ hyalronidase,hyl）毒力基因的克隆复合群17（clonal complex 17, CC17）屎肠球菌。结论河南睢县3岁以下儿童携带屎肠球菌对多种抗生素广泛耐药,耐药菌株中存在携带毒力基因的CC17克隆群的菌株。Objective To investigate the antibiotic resistance, virulence genes, and sequence types of Enterococcus faecium （ E. faecium） strains isolated from children under 3 years old in Sui county, Henan province. Methods Kirby-Bauer antibiotic testing was performed to analyze the antibiotic sensitivi-ties of E. faecium strains to 15 common antibiotics. PCR analysis was used to detect the virulence genes car-ried by the E. faecium strains. Multilocus sequence typing （ MLST） was performed for the typing of E. faeci-um strains. Results Forty-seven E. faecium strains were isolated from 120 stool samples collected from chil-dren under 3 years old in Sui county, Henan province, of which 95. 7% were antibiotic-resistant strains. Most of the isolated E. faecium strains were resistant to rifampicin, accounting for 91. 5% （43/47） of all isolates, followed by those resistant to erythromycin and tetracycline, which accounted for 68. 1% （32/47）. Moreover, high resistance rates to those antibiotics commonly used in clinical treatments of E. faecium infec-tion including β-lactam and aminoglycosides antibiotics were observed. Those strains resistant to more than three kinds of antibiotics belonged to the same clonal complex including 12 strains of clonal complex 17 （ CC17） harboring the virulence gene of hyl. All of the isolated E. faecium strains were susceptible to vanco-mycin, linezolid, chloromycetin and nitrofurantoin. Conclusion The E. faecium strains isolated from chil-dren under 3 years old in Sui county, Henan province were multi-antibiotic resistant. There were drug-resist-ance strains belonging to the CC17 and carrying the virulence gene of hyl.

关 键 词：屎肠球菌 耐药性 毒力基因 MLST分析 

分 类 号：R446.5[医药卫生—诊断学] R440[医药卫生—临床医学]