天然提取物对γ-氨基丁酸转运体功能的作用筛选  

The screening of natural extracts effects on the activity ofγ-aminobutyric acid transporters( GATs)

作　　者：胡静[1] 张劲松[2] 樊华[3] 

机构地区：[1]江南大学无锡医学院,江苏无锡214122 [2]国家食用菌工程技术研究中心国家食用菌加工技术研发分中心上海市农业科学院食用菌研究所,上海201403 [3]柏林自由大学夏洛特医学院,德国柏林13353

出　　处：《食品与发酵工业》2016年第9期87-90,103,共5页Food and Fermentation Industries

基　　金：江苏省自然科学基金(BK20150140)

摘　　要：γ-氨基丁酸(γ-aminobutyric acid,GABA)是哺乳动物中枢神经系统中主要的抑制性神经递质。GABA转运蛋白(GABA transporters,GATs)通过摄取释放的GABA有效控制其在突触间隙的浓度,从而成为控制GABA能的信号传导的关键因素。大量研究表明:灵芝、虎杖等传统中草药的提取成分具有抗肿瘤、免疫调节、保护心血管系统等药理作用。该研究以稳定表达GABA转运蛋白(GAT1)的CHO细胞为模型,利用GABA摄取的定量分析测试来初步研究灵芝粗提物和虎杖主要有效成分白藜芦醇对GABA转运体功能的影响。白藜芦醇通过典型的非竞争性抑制的方式影响GAT1蛋白摄取GABA的功能。GABA（ γ-aminobutyric acid） is the major inhibitory neurotransmitter in the central nervous system（ CNS）. GABA transporters（ GATs） are key elements in the termination of the synaptic actions of GABA by re-uptake GABA,which is one important mechanism in the regulation of GABA concentration in the synaptic cleft. Various studies have showed that extracts from the traditional Chinese medicines such as Ganoderma lucidum and Polygonum cuspidatum have certain improved effect on anti-tumor activity,regulation of immune system andcardiovascular systems. In this study,the effect on GABA uptake activity of GAT1 of crude extracts from Ganoderma lucidum and resveratrol by the effective component of Polygonum cuspidatum were tested in CHO / GAT1 cells by GABA uptake assay.Resveratrol was found as a typical non-competitive inhibition on GABA uptake of GAT1.

关 键 词：Γ-氨基丁酸 GABA转运蛋白 灵芝 白藜芦醇 

分 类 号：R285[医药卫生—中药学]