Novel Gelatin-based Nano-gels with Coordination-induced Drug Loading for Intracellular Delivery  

作　　者：Changjiang Fan Dong-An Wang 

机构地区：[1]Division of Bioengineering, School of Chemical and Biomedical Engineering, Nanyang Technological University [2]Institute for Translational Medicine, College of Medicine, Qingdao University

出　　处：《Journal of Materials Science & Technology》2016年第9期840-844,共5页材料科学技术（英文版）

基　　金：supported by the AcRF Tier 2 ARC 1/13 of the Ministry of Education, Singapore;the National Natural Science Foundation of China (No. 51328301)

摘　　要：In this study, we develop the gelatin-dopamine （Gel-Dopa） nano-gels （GDNGs） and explore their potential as drug delivery vehicles. The GeI-Dopa precursor is synthesized using EDC/NHS coupling reaction, in which the catechols can coordinate with transition metal ions such as Fe^3＋. These novel GDNGs exhibit excellent cytocompatibility. The model drug, doxorubicin （Dox）, is readily conjugated into catechol of GDNGs by the coordination cross-link of Fe^3＋ ion. The morphology and size distribution of the nanogels are characterized via field emission scanning electron microscopy and particle size analyzer, respectively. The GDNGs loaded with Dox （GDNGs-Dox） is capable of efficiently penetrating ceil membrane and enter the HeLa cells. The endocytosed GDNGs-Dox release Dox molecules and subsequently kill the tumor cells.In this study, we develop the gelatin-dopamine （Gel-Dopa） nano-gels （GDNGs） and explore their potential as drug delivery vehicles. The GeI-Dopa precursor is synthesized using EDC/NHS coupling reaction, in which the catechols can coordinate with transition metal ions such as Fe^3＋. These novel GDNGs exhibit excellent cytocompatibility. The model drug, doxorubicin （Dox）, is readily conjugated into catechol of GDNGs by the coordination cross-link of Fe^3＋ ion. The morphology and size distribution of the nanogels are characterized via field emission scanning electron microscopy and particle size analyzer, respectively. The GDNGs loaded with Dox （GDNGs-Dox） is capable of efficiently penetrating ceil membrane and enter the HeLa cells. The endocytosed GDNGs-Dox release Dox molecules and subsequently kill the tumor cells.

关 键 词：Gelatin Dopamine Doxorubicin Coordination interaction Drug delivery 

分 类 号：R943[医药卫生—药剂学] O648.17[医药卫生—药学]