Influence of 3D Microgrooves on C2C12 Cell Proliferation,Migration,Alignment,F-actin Protein Expression and Gene Expression  

作　　者：Huichang Gao 

机构地区：[1]Department of Biomedical Engineering,School of Materials Science and Engineering,South China University of Technology,Guangzhou 510641,China [2]National Engineering Research Center for Tissue Restoration and Reconstruction,Guangzhou 510006,China [3]Guangdong Province Key Laboratory of Biomedical Engineering,South China University of Technology,Guangzhou 510006,China

出　　处：《Journal of Materials Science & Technology》2016年第9期901-908,共8页材料科学技术（英文版）

基　　金：supported by the National Basic Research Program of China(Nos.2012CB619100 and 2011CB606204);the National Natural Science Foundation of China(Nos.51373056,51232002 and 51372085);the“111 Project”(No.B13039);the Fundamental Research Funds for the Central Universities

摘　　要：In this paper, we fabricated three kinds of 3D microgrooves with different depth on biocompatible poly（lactic-co-glycolic acid） （PLGA） substrate via combination of soft-lithography and melt-casting methods, and investigated in detail their influence on C2C12 cell behaviors. It is found that cell proliferation, migration, alignment, spatial distribution, F-actin protein expression and gene expression are all remarkably distinct on these microgrooved samples and the smooth control PLGA substrate. The associated underlying mechanisms were further analyzed and discussed using real-time living cell monitoring, confocal laser scanning microscopy and gene microarray. Our preliminary results suggested that 3D microstruc- ture could affect cell behaviors in a much more extensive manner than what we had understood before.In this paper, we fabricated three kinds of 3D microgrooves with different depth on biocompatible poly（lactic-co-glycolic acid） （PLGA） substrate via combination of soft-lithography and melt-casting methods, and investigated in detail their influence on C2C12 cell behaviors. It is found that cell proliferation, migration, alignment, spatial distribution, F-actin protein expression and gene expression are all remarkably distinct on these microgrooved samples and the smooth control PLGA substrate. The associated underlying mechanisms were further analyzed and discussed using real-time living cell monitoring, confocal laser scanning microscopy and gene microarray. Our preliminary results suggested that 3D microstruc- ture could affect cell behaviors in a much more extensive manner than what we had understood before.

关 键 词：Cell behaviors3D microgroove Poly（lactic-co-glycolic acid）Cytoskeleton Gene microarray Real-time living cell monitoring 

分 类 号：Q253[生物学—细胞生物学] Q78