赤霉素处理对重楼种子萌发相关生理指标的影响  

Effect of Exogenous Gibberellic Acid on the Physiological and Biochemical Factors in the Germination Process of Paris polyphylla var. chinensis seeds

作　　者：宋发军[1] 罗忠[1] 黄珍[1] 孟艳艳[1] 张鹏[1] 

机构地区：[1]中南民族大学生命科学学院,生物技术国家民委重点实验室/武陵山区特色资源植物种质保护与利用湖北省重点实验室,武汉430074

出　　处：《中南民族大学学报（自然科学版）》2016年第3期30-34,共5页Journal of South-Central University for Nationalities：Natural Science Edition

基　　金：国家自然科学基金资助项目(31370118);中南民族大学基本科研业务费专项资金资助项目(CZW15019);中南民族大学大学生创新创业资助项目(GCX15006;SCX15004);校企合作项目(HZY14024);湖北省生物技术专业综合改革试点项目(GJZ15006)

摘　　要：为分析赤霉素(GA_3)处理对重楼种子萌发相关生理指标的影响,检测了500 mg/L外源GA_3处理以及对照处理的华重楼种子萌发过程中SOD、POD、CAT和G6PDH酶活性以及内源激素ZT、GA_3、IAA和ABA含量的变化.结果表明:在90 d检测范围内,GA_3处理种子的SOD活性达到最大值的时间(第30 d)早于对照组(第60 d),CAT酶活性最高峰值高于对照组;而且,除第0 d样品外,GA_3处理种子的G6PDH酶活性、CAT酶活性均大于对照组;GA_3处理种子的ZT含量、内源GA_3含量和IAA含量的最高峰值及其出现时间均高于和早于对照组;POD酶活性则均低于对照;GA_3处理种子的ABA含量比对照下降幅度更大,最低值更小.可见500 mg/L外源GA_3处理促进了华重楼种子中SOD、CAT、G6PDH酶活性以及ZT、内源GA_3和IAA含量的增加,降低了POD酶活性以及ABA含量.In order to reveal the influence of exogenous Gibberellic acid （ GA3） on the physiological and biochemical factors in the germination process of P. polyphylla seeds, the activity of S O D , POD , CAT , G6PDH and the content of ZT, endogenous GA3,IAA,ABA were detected in the germination process of P. polyphylla seeds treated with 500 m g / L endogenous GA3 and mocked-treated with H20 as control. Results In the 90 days range, the appearance time of the highest SOD activity in GA3-treated seeds （day 30） was earlier than the control （day 60） , the highest CAT activity in GA3-treated seeds was higher than the control, and the activity of G6PDH and CAT in GA3-treated seeds was higher than the control except in the day 0 samples. The highest content and its appearance time of ZT, endogenous GA3, IAA in GA3 -treated seeds were higher and earlier than the results of the control. However, the POD activity in GA3-treated seeds was lower than that of the control, and the decreasing extent and the minimum content of ABA in GA3 -treated seeds was more significant and lower than the results of the control. Conclusions： Treatment with 500 m g / L exogenous GA3 led to increase the activity of SOD , CAT, G6PDH and the content of ZT, endogenous GA3, IAA, in the meanwhile, and decrease the POD activity and the ABA content in GA3-treated P. polyphylla seeds.

关 键 词：华重楼 种子萌发 赤霉素 生理生化指标 

分 类 号：Q945.4[生物学—植物学]