碳点-荧光素荧光共振能量转移体系在阿司匹林测定中的研究与应用  

Study on Carbon Dots-Fluorescein Fluorescence Resonance Energy Transfer System and Its Application in Aspirin Analysis

作　　者：金文英[1] 廖秀芬 陶慧林[1] 赵颖 刘绍州 梁永敏 

机构地区：[1]广西高校食品安全与检测重点实验室桂林理工大学化学与生物工程学院,广西桂林541004 [2]广西产品质量检验研究院,广西南宁530007

出　　处：《分析测试学报》2016年第9期1147-1151,共5页Journal of Instrumental Analysis

基　　金：国家自然科学基金资助项目(61264007);广西高校食品安全与检测重点实验室资助项目

摘　　要：研究了经L-y半胱氨酸修饰后的碳点（CDs）-荧光素（FAM）荧光共振能量转移体系，并利用该体系建立了测定阿司匹林（ASP）的新方法。结果表明：在λex=330 nm下，于pH 7.0的Tris-HCl缓冲液中，CDs与FAM反应5 min后能发生有效的荧光共振能量转移，能量供体CDs将能量转移到受体FAM，使FAM的荧光显著增强，而ASP的加入可有效猝灭FAM的荧光，且ASP浓度在1.0~150.0 μg/mL范围内与体系的荧光猝灭值ΔIF呈良好的线性关系（r=0.999 6），基于此建立了测定ASP的新方法。在最佳实验条件下，方法的检出限达0.33 μg/mL（3δ/k,n=11），回收率为 97.1%~105.3%，相对标准偏差（RSD）不大于4.6%（n=6）。常见的无机离子以及与ASP同类型的药物对测定影响较小，方法的选择性较好。A fluorescence resonance energy transfer（FRET）system between fluorescence carbon dots（CDs,donor）and fluorescein（FAM,acceptor）was constructed,and a novel method for the sensitive and selective determination of aspirin（ASP）was accordingly proposed.It was found that in pH 7.0 Tris-HCl buffer，effective energy transfer from CDs to FAM occurred,which resulted in a great enhancement of the fluorescence intensity of FAM.Upon the addition of ASP,fluorescence quenching of FAM occurred,and the change of FAM fluorescence intensity was in a good linearity（r=0.999 6）with ASP in the concentration range of 1.0-150.0 μg/mL.A novel fluorescence quenching platform for the determination of ASP was constructed.Under the optimum conditions,the detection limit of ASP for this method was 0.33 μg/mL（3δ/k,n=11）,and the spiked recoveries ranged from 97.1%to 105.3%with RSD（n=6）not more than 4.6%.The result showed that common relevant substance,cations and anions did not interfere with the detection of ASP.

关 键 词：碳点 荧光素 荧光共振能量转移(FRET) 阿司匹林 

分 类 号：O657.3[理学—分析化学] TQ460.72[理学—化学]