基于离子交换-电导检测法对酿酒葡萄中有机酸含量进行分析  

Determination of Organic Acid in Wipe Grapes Based on Ion Exchange-conductivity Detection Method

作　　者：杨春霞[1] 

机构地区：[1]宁夏农林科学院农产品质监中心,宁夏银川750002

出　　处：《分析测试学报》2016年第9期1162-1166,共5页Journal of Instrumental Analysis

基　　金：宁夏回族自治区自然基金项目资助(NZ14197)

摘　　要：建立了离子色谱法测定酿酒葡萄中乳酸、丙酸、苹果酸、酒石酸、草酸、柠檬酸、琥珀酸和抗坏血酸的方法。样品经提取、脱色、过滤后以自动淋洗液发生器生成的1.5~30 mmol/L KOH为淋洗液洗脱，抑制电导检测器检测。考察了共存非测定离子及相邻离子间的干扰，结果表明非测定无机离子对测定无干扰。8种有机酸在一定浓度范围内与其峰面积呈良好的线性关系，检出限为0.064~0.25 mg/L，加标回收率为89.5%~96.2%。该方法用于宁夏贺兰山东麓酿酒葡萄中8种有机酸分析，8种有机酸均被检出，以苹果酸和酒石酸含量最高，柠檬酸次之，其它酸含量均较低。酿酒葡萄为典型的酒石酸型水果。A novel ion chromatographic method was developed for the determination of organic acids in wipe grapes,including lactic acid,propionic acid，DL-malic acid，D-tartaric acid，oxalic acid，citrate，succinic acid and ascorbic acid.The samples were prepared through extraction decoloration and filtration before analysis,and eluted with 1.5-30 mmol/L KOH produced by an eluent antogenerator.The interference between the coexistence ions which were not examined and adjacent ions was investigated.The results showed that the unexamed inorganic ions made no interference on determination.The eight organic acids showed good linear relationship between mass concentration and peak area in the certain concentration ranges.The detection limits were in the range of 0.064-0.25 mg/L and the average recoveries were 89.5%-96.2%.The method was applied in the determination of eight organic acids in wine grapes from eastern Helan mountain，Ningxia.The results showed that eight organic acids were all detected in wine grapes with high contents of DL-malic acid and D-tartaric acid，citric acid，and low contents of other acids.The wine grapes is a typical tartaric acid fruit.

关 键 词：离子交换-电导检测法 有机酸 酿酒葡萄 

分 类 号：O657.75[理学—分析化学] Q946.81[理学—化学]