七氟烷对低温环境下失血性休克猪的心肌保护作用  

The protective effect of sevoflurane on the myocardium of pig with hemorrhagic shock in environmental hypothermia

作　　者：孙佳[1] 邹彬[2] 于冬梅[2] 张铁铮[2] 

机构地区：[1]辽宁医学院沈阳军区总医院研究生培养基地,辽宁沈阳110016 [2]沈阳军区总医院麻醉科,辽宁沈阳110016

出　　处：《沈阳药科大学学报》2016年第9期735-740,758,共7页Journal of Shenyang Pharmaceutical University

基　　金：军队后勤科研重点资金资助项目(BWS12J008)

摘　　要：目的 探讨七氟烷对低温环境下失血性休克猪的心肌保护作用。方法 巴马小型猪32头,雌雄不限,体质量20～25 kg,按随机数字表法分为4组,每组8头。分别为假手术组（S组）、失血性休克组（HS组）、七氟烷预处理组（Pre/Sev组）和七氟烷后处理组（Post/Sev组）。监测并记录各组平均动脉压、心率和中心体温,各组分别于休克前及休克后30、60、90、120、180和240 min共7个时间点采集血液,采用ELISA法检测血清中白细胞介素-6（interleukin 6,IL-6）、肿瘤坏死因子-α（tumour necrosis factorα,TNF-α）和超氧化物歧化酶（superoxide dismutase,SOD）的含量。结果 与S组比较,HS组、Pre/Sev组和Post/Sev组血浆中TNF-α和IL-6含量在休克后60 min和90 min显著升高（P〈0.05）,其中TNF-α含量在休克120 min时达到高峰,休克180 min时显著低于120 min（P〈0.05）;血清中SOD含量显著降低（P〈0.05）。与HS组比较,Pre/Sev组和Post/Sev组血浆中TNF-α和IL-6含量显著降低（P〈0.05）;血清中SOD含量显著升高（P〈0.05）。Pre/Sev组和Post/Sev组各项比较差异无显著意义（P〉0.05）。结论 七氟烷对低温环境下失血性休克猪心肌具有保护作用,其主要机制与抑制炎症反应,增强机体抗氧化能力有关。Objective To investigate the protective effect of sevoflurane on the myocardium of pig with hemorrhagic shock （HS）in environmental hypothermia. Methods Thirty-two Bama miniature pigs were randomly divided into four groups with eight animals for each group： sham group （group S）, hemorrhagic shock group （group HS ）, sevoflurane pre-conditioning group （group Pre/Sev ）and sevoflurane post- conditioning group（ group Post/Sev）. At the time points before HS （to ）, and 30,60,90,120,180 and 240 min after HS （ corresponding to t30 t60 t90 t120 t180 and t240 respectively ）, mean arterial pressure （ MAP）, heart rate （HR）and core temperature were monitored and recorded for every group, and at the same time, blood samples were collected, centrifuged and frozen until analysis for concentration of interleukin 6 （ IL-6 ）, tumour necrosis factor a （TNF-a） and superoxide dismutase （SOD） in serum. Results Compared with group S, the concentration of TNF-a and IL-6 in serum in group HS, Pre/Sev and Post/Sev was increased at 60 or 90 rain after HS（P 〈0. 05） and The TNF-α reached the maximum at 120 min after HS and decreased after HS 180 min（P 〈0. 05） while SOD in serum in group HS,Pre/Sev and Post/Sev decreased obviously（ P 〈 0. 05 ）. Compared with group HS ,TNF-α and IL-6 in serum of group Pre/Sev and Post/Sev was decreased （ P 〈 0.05 ） and SOD in serum of group Pre/Sev and Post/Sev was increased obviously （ P 〈 0.05 ）. There was no significant difference between group Pre/Sev and Post/Sev（ P 〉 0. 05 ）. Conclusions Sevoflurane has a protective effect on the myocardium of pig with hemorrhagic shock in environmental hypothermia, and the main mechanism may be related to attenuate inflammatory response and enhance the antioxidant capacity.

关 键 词：七氟烷 环境低温 失血性休克 心脏保护 白细胞介素-6 肿瘤坏死因子-α 氧化应激 小型猪 

分 类 号：R96[医药卫生—药理学]