耐酸性α-淀粉酶产生菌的筛选及其产酶条件优化  

Screening and optimization of acid resistant strain producing a-amylase

作　　者：任世英[1] 邵奎[1] 李雯[1] 张威[1] 刘飞[2] 

机构地区：[1]淮阴工学院生命科学与食品工程学院,江苏淮安223003 [2]淮阴工学院化学工程学院,江苏淮安223003

出　　处：《中国饲料》2016年第17期10-12,共3页China Feed

基　　金：国家自然科学基金项目(51308245);江苏省淮安市科技计划项目(HAN2015026);江苏省生物质转化与过程集成工程实验室开放课题(JPELBCPL2013004和JPELBCPL2014006)

摘　　要：为了分离得到饲料用耐酸性α-淀粉酶的菌株,从食堂排污口取土样,筛选到一株耐酸(p H=4)且产α-淀粉酶的菌株,并对其形态特征、培养特征、生理生化特征、酶学性质及产酶条件进行研究。结果表明:菌体呈杆状,革兰氏染色阳性,胞内形成异染粒和PHB,接触酶、淀粉水解、卵磷脂酶试验呈阳性,反硝化试验、荧光色素呈阴性。根据菌体形态、菌落特征及部分生理生化特性,将其初步鉴定为芽孢杆菌属,即Bacillus sp.SD-1。通过单因素试验对菌株的最适产酶温度及p H进行优化,培养温度为43℃,p H为4.5,发酵60 h,酸性α-淀粉酶活力达到74.6 U/m L。In order to obtain acid resistant strains producing α-amylase for the application in feed industrial field. Clear circle was used to initially screen desired strains at pH 4.SD-1 was chosen as experimental strain owning to its higher ratio of dissolving circle diameter to colony diameter.Strain SD-1 was rod , G＋ .Test results of metachromatic granule staining,PHB,catalase,amylolysis,lecithinase were positive.Test results of denitrification and fluorchrome were negative. According to its cell and colony morphology ,physiological and biochemical properties, strain SD-1 belongs to genus Bacillus,namely Bacillus sp.SD-1.Optimization of higher α-amylase production conditions：43 ℃,pH 4.5 ,fermentation for 60 h, the activity of α-amylase was up to 74.6 U/mL.

关 键 词：耐酸性 a-淀粉酶产生菌 芽孢杆菌属 

分 类 号：S816.6[农业科学—饲料科学]