IGRP_(206-214)dtSCT四聚体偶联皂草素的制备及初步应用  

The preparation and preliminary application of Saporin-Coupled dtSCT IGRP_(206-214) H-2K^d in NOD mice

作　　者：王宇航[1] 窦延[1] 徐娅雯[1] 马旻轩 李凯[1] 潘兴元[1] 钱莉[1] 龚卫娟[1] 季明春[1] 

机构地区：[1]扬州大学医学院,江苏扬州225001

出　　处：《扬州大学学报（农业与生命科学版）》2016年第2期12-16,共5页Journal of Yangzhou University：Agricultural and Life Science Edition

基　　金：国家自然科学基金资助项目(30771955);江苏省高校研究生科技创新计划项目(CXLX12-0939)

摘　　要：以InsB15-23dtSCT原核表达载体为基础,制备dtSCT型IGRP206-214H-2Kd原核表达载体;将获取的融合蛋白IGRP206-214H-2Kd单体,生物素化制成IGRP206-214dtSCT四聚体,进一步将皂草素与IGRP206-214dtSCT四聚体偶联;流式检测皂草素偶联IGRP206-214dtSCT四聚体与NOD鼠脾脏淋巴细胞共孵育体系中IGRP206-214特异性细胞毒性T细胞(CTL)。结果表明:皂草素偶联IGRP206-214dtSCT四聚体与NOD鼠脾脏淋巴细胞共孵育后,IGRP206-214特异性CTL的频率与PBS对照组相比明显下降(P<0.001)。说明偶联皂草素的IGRP206-214dtSCT四聚体在体外能够靶向清除NOD鼠抗原特异性CTL。ABSTRACT： On the basis of InsB15 23 dtSCT prokaryotic expression vector, dtSCT type of IGRP206-214 H-2Kd prokaryotic expression vector was eonstruet. The obtained fusion protein IGRP20G-214 H-2Kd monomer was biotinylated, then the IGRP206 214 dtSCT tetramer was coupled with saporin. The spleen lymphoeytes from NOD were co-cultured with saporin- Coupled dtSCT IGRP206-214 H-2Kd tetramer and the IGRP206- 214-speeific CTLs were detected by Flow Cytometry. Co-incu- bated NOD spleen lymphocytes together with Saporin Coupled dtSCT IGRP206- 214 H-2Kdtetramer, the IGRP206-214-specifie CTLs significantly decreased compared to the PBS control group （P（0. 001, n= 6）. These results showed that Saporin Coupled dtSCT IGRP206-214 H-2Kd tetramer can selectively clear NOD specific CTLs in vitro.

关 键 词：H-2Kd四聚体 CD8+T细胞 1型糖尿病 NOD鼠 

分 类 号：R392.2[医药卫生—免疫学]