1个新水稻黄叶突变体yl1的遗传分析和初步定位  

Genetic analysis and gene mapping of a novel yellow leaf mutant yl1 in rice(Oryza sativa L.)

作　　者：胡智[1] 何蔷薇 郭旻[1] 李荣德[1] 朱茜[1] 刘敏[1] 严长杰[1] 

机构地区：[1]扬州大学农学院/江苏省作物遗传生理国家重点实验室培育点/粮食作物现代产业技术协同创新中心,江苏扬州225009

出　　处：《扬州大学学报（农业与生命科学版）》2016年第2期51-55,共5页Journal of Yangzhou University：Agricultural and Life Science Edition

基　　金：国家自然科学基金资助项目(31171158);农业部转基因专项(2014ZX08009-003-005);江苏省自然科学基金资助项目(BK2012684);江苏省普通高校研究生科研创新计划项目(CXLX_1418);江苏省六大人才高峰项目(2012-NY-027);江苏高校优势学科建设工程项目(2014-12)

摘　　要：在籼稻蜀恢498群体中发现1个黄叶自然突变体(yl1)。农艺性状调查发现,与野生型相比,突变体的株高、千粒重、结实率和有效穗数均明显降低。叶绿素含量测定表明,突变体yl1在苗期叶绿素含量极显著降低。超显微结构观察发现,突变体叶绿体形态改变与野生型相比明显变小,类囊体变小且不规则。进一步通过遗传分析表明该黄叶突变体受1个隐性单基因控制,利用SSR和STS标记定位目的基因YL1,初步定位将YL1在水稻第1号染色体长臂上Y99和Y33之间,物理距离大约157kb区段内。在该区段内未见相关基因报道,推测YL1是控制叶色的新基因。In the present study, we found a spontaneous mutant characterized by yellow leaves （named as yll） from an indica eultivar Shuhui498. Morphological analysis showed that, the yll mutant exhibited shorter plant height and decreased 1000-grain weight, seed setting rate and the effective panicles compared to its wild type. Moreover, the chloro- phyll content in the yll mutant leaves was found to be dramatically decreased at the seedling stage. And the SEM result also showed that the chloroplast in the yll mutant morphologically changed, and the thylakoid was smaller and irregular. Genetic analysis showed that the yll mutant was controlled by a single recessive gene. By using SSR and STS markers, the YL1 gene was located betweenY99 and Y33 with a physical distance of 157 kb on the long arm of ehromosome 1. The YL 1 gene is considered as a novel gene controlling the leaf color in rice.

关 键 词：水稻 黄叶 遗传分析 基因定位 

分 类 号：S511[农业科学—作物学]