高效液相色谱法同时测定四逆散中6种抗抑郁成分含量  

Simultaneous determination of six kinds of antidepression components in Sinisan by HPLC

作　　者：宗阳[1] 孙冰婷 朱立静[1] 张倩[1] 居文政[1] 

机构地区：[1]南京中医药大学附属医院临床药理科,江苏南京210029

出　　处：《中国医院药学杂志》2016年第17期1509-1512,共4页Chinese Journal of Hospital Pharmacy

基　　金：国家自然科学基金资助项目(编号:81573685;重大新药创制(编号:2012ZX09303009-002)

摘　　要：目的：建立高效液相色谱法同时测定四逆散中6种抗抑郁成分：柴胡皂苷A、芍药苷、橙皮苷、柚皮苷、新橙皮苷和甘草苷含量的方法。方法：采用高效液相色谱法,色谱柱为Agilent Zorbax C18色谱柱（4.6 mm×150 mm,5μm）,流动相为水（A）-乙腈（B）,梯度洗脱,流速为1.0mL·min-1,柱温为30℃,检测波长为203 nm,进样量为20μL。结果：柴胡皂苷A、芍药苷、橙皮苷、柚皮苷、新橙皮苷和甘草苷的线性范围分别为2.12~33.86μg·mL-1（r2=1.0000）、5.91~94.60μg·mL-1（r2=0.999 9）、2.12~33.86μg·mL-1（r2=0.999 9）、7.26~116.2μg·mL-1（r2=1.0000）、13.5~216.0μg·mL-1（r2=0.999 9）、2.02-32.33μg·mL-1（r2=0.999 9）。精密度、重复性和稳定性试验RSD均符合要求。柴胡皂苷A、芍药苷、橙皮苷、柚皮苷、新橙皮苷和甘草苷的加样回收率分别为102.77%、101.49%、101.18%、101.26%、99.06%。结论：本法灵敏简便、准确度高,适用于同时测定四逆散中6种抗抑郁成分柴胡皂苷A、芍药苷、橙皮苷、柚皮苷、新橙皮苷和甘草苷的含量,可用于四逆散的质量控制。ABSTRACT： OBJECTIVE A HPLC method was established to determinate Saikosaponin A, Paeoniflorin, Aurantiamarin, Na- ringin, Neohesperidin and Liquiritin in Sinisan. METHODS Analysis was conducted by HPLC-DAD on Agilent Zorbax C18 （4.6 mm×150 mm,5μm）column with mobile phase of water （A）- acetonitrile （B）at the flow rate of 1.0 mL.min^-1 by gradi-ent elution. The de-tection wavelengths were at 203 nm, Injection volume：20μL. RESULTS The linear range of Saikosaponin A, Paeoniflorin, Aurantiamarin, Naringin, Neohesperidin and Liquiritin were 2. 12 -33. 86μg.mL-1 （r2 = 1. 000 0）,5. 91 - 94. 60μg·mL^-1 （r2 =0. 999 9）, 2. 12- 33.86 μg.mL-1 （r2 = 0. 999 9）, 7. 26- 116. 2 /μg-mL^-1 （r2 = 1. 000 0）, 13.5 - 216. 0μg.mL^-1 （r2 = 0. 999 9）, 2. 02 - 32. 33μg·mL^-1 （r2 = 0. 999 9） respectively. Precision, reproducibility and stability RSD all met the requirements. The average recoveries of saikosaponinA, paeoniflorin, aurantiamarin, naringin, neohesperidin and liquiritinwere99.59%, 102.77%, 101.49%, 101.18%, 101.26%, 99.06%o respectively. CONCLUSION The established method was convenient, accurate and suitable for simultaneous determination of saikosaponin A, paeoniflorin, a-urantiamarin, naringin, neohesperidin and liquiritin in Sinisan.

关 键 词：四逆散 抗抑郁 柴胡皂苷A 芍药苷 橙皮苷 柚皮苷 新橙皮苷 甘草苷 高效液相色谱法 

分 类 号：R917[医药卫生—药物分析学]