Pharmacokinetic profiles of falcarindiol and oplopandiol in rats after oral administration of polyynes extract of Oplopanax elatus  

作　　者：SUN Wei HE Yi-Sheng XU Ling-Hui ZHANG Bi-Ying QI Lian-Wen YANG Jie LI Ping WEN Xiao-Dong 

机构地区：[1]State Key Laboratory of Natural Medicines, China Pharmaceutical University

出　　处：《Chinese Journal of Natural Medicines》2016年第9期714-720,共7页中国天然药物（英文版）

基　　金：supported by National Natural Science Foundation of China(Nos.81373957,81373919,and 81573567);Natural Science Foundation of Jiangsu Province(No.BK20131306);Jiangsu"Shuang Chuang"Team Program

摘　　要：Polyynes, such as facarindiol(FAD) and oplopandiol(OPD), are responsible for anticancer activities of Oplopanax elatus(O. elatus). A novel approach to pharmacokinetics determination of the two natural polyynes in rats was developed and validated using a liquid chromatography-electrospray ionization-mass spectrometry(LC-MS) method. Biosamples were prepared by liquid-liquid extraction using ethyl acetate/n-hexane(V : V = 9 : 1) and the analytes were eluted on an Agilent ZORBAX Eclipse Plus C18 threaded column(4.6 mm × 50 mm, 1.8 μm) with the mobile phase of acetonitrile-0.1% aqueous formic acid at a flow-rate of 0.5 m L·min–1 within a total run time of 11 min. All analytes were simultaneously monitored in a single-quadrupole mass spectrometer in the selected ion monitoring(SIM) mode using electrospray source in positive mode. The method was demonstrated to be rapid, sensitive, and reliable, and it was successfully applied to the pharmacokinetic studies of the two polyynes in rat plasma after oral administration of polyynes extract of O. elatus.Polyynes, such as facarindiol（FAD） and oplopandiol（OPD）, are responsible for anticancer activities of Oplopanax elatus（O. elatus）. A novel approach to pharmacokinetics determination of the two natural polyynes in rats was developed and validated using a liquid chromatography-electrospray ionization-mass spectrometry（LC-MS） method. Biosamples were prepared by liquid-liquid extraction using ethyl acetate/n-hexane（V ： V = 9 ： 1） and the analytes were eluted on an Agilent ZORBAX Eclipse Plus C18 threaded column（4.6 mm × 50 mm, 1.8 μm） with the mobile phase of acetonitrile-0.1% aqueous formic acid at a flow-rate of 0.5 m L·min–1 within a total run time of 11 min. All analytes were simultaneously monitored in a single-quadrupole mass spectrometer in the selected ion monitoring（SIM） mode using electrospray source in positive mode. The method was demonstrated to be rapid, sensitive, and reliable, and it was successfully applied to the pharmacokinetic studies of the two polyynes in rat plasma after oral administration of polyynes extract of O. elatus.

关 键 词：Oplopanax elatus POLYYNES LC-MS Pharmacokinetic study 

分 类 号：R917[医药卫生—药物分析学]