黄嘌呤和鸟嘌呤在石墨烯-聚唑类化合物修饰电极上电化学行为  

Graphene/azole organic polymer modified glassy carbon electrode for simultaneous determination of xanthine and guanine in urine

作　　者：陈宗保[1] 刘林海[1] 董洪霞[1] 

机构地区：[1]上饶师范学院,江西省普通高校应用有机化学重点实验室,上饶334001

出　　处：《分析试验室》2016年第9期998-1001,共4页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金(21465020);江西省教育厅科技项目(151057)资助

摘　　要：采用滴涂法和循环伏安法制备石墨烯-聚唑类化合物复合膜修饰玻碳电极,分别用循环伏安法和差分脉冲伏安法研究黄嘌呤和鸟嘌呤混合物在修饰电极上的电化学行为。在0.2 mol/L的磷酸盐缓冲液(p H 5.5)中,黄嘌呤和鸟嘌呤混合物在修饰的玻碳电极上具有较好的电化学行为。鸟嘌呤和黄嘌呤分别在4.0×10^(-4)~4.0×10^(-6)mol/L和4.0×10^(-5)~4.0×10^(-8)mol/L范围内有较好的电化学响应,鸟嘌呤和黄嘌呤检出限分别为4.0×10^(-7)mol/L和4.0×10^(-9)mol/L。方法用于同时测定人体尿液中鸟嘌呤和黄嘌呤,回收率分别为102.1%~105.9%和96.9%~106.5%。The glassy carbon electrode was modified by graphene and azole organic polymer in this study. The electrochemical behavior of xanthine and guanine was characterized by the cyclic voltammetry and differential pulse voltammetry at the modified electrode. The results showed that xanthine and guanine had excellent electrochemical behavior at the modified electrode with 0. 20 mol/L phosphate buffer solution（p H 5. 5）.Guanine and xanthine had good electrochemical response in the range of 4. 0 × 10^-4~ 4. 0 × 10^-6mol/L and 4. 0× 10^-5~ 4. 0 × 10^-8mol/L,respectively. The detection limit of two analytes were 4. 0 × 10^-7mol/L and 4. 0 ×10^-9mol/L,respectively. The proposed method was used to the analysis of human urine samples and the recoveries were in the range of 102. 1% ~ 105. 9 % and 96. 9% ~ 106. 5%

关 键 词：石墨烯 4-氨基-4H-1 2 4 -三唑 黄嘌呤 鸟嘌呤 差分脉冲伏安法 

分 类 号：O657.1[理学—分析化学]