山羊繁殖季节性相关基因DIO2与DIO3的表达分析  

Expression Analysis of DIO2 and DIO3 Genes Related to Reproductive Seasonality in Goats(Capra hircus)

作　　者：黄冬维[1,2] 狄冉[1] 胡文萍[1] 王翔宇[1] 潘章源[1] 郭晓飞[1] 曹晓涵[1] 刘秋月[1] 储明星[1] 

机构地区：[1]中国农业科学院北京畜牧兽医研究所/农业部畜禽遗传资源与种质创新重点实验室,北京100193 [2]安徽省农业科学院畜牧兽医研究所,合肥230031

出　　处：《农业生物技术学报》2016年第10期1536-1543,共8页Journal of Agricultural Biotechnology

基　　金：国家自然科学基金(No.30871773);国家转基因科技重大专项(No.2016ZX08009-003-006和No.2016ZX08010-005-003);国家肉羊产业技术体系专项(No.CARS-39);中国农业科学院科技创新工程(No.ASTIP-IAS13)

摘　　要：研究表明,2型脱碘酶基因(typeⅡiodothyronine deiodinase gene,DIO2)和3型脱碘酶基因(typeⅢiodothyronine deiodinase gene,DIO3)对啮齿动物(Rodentia)和绵羊(Ovis aries)的季节性繁殖活动具有重要调控作用。本研究选择常年发情济宁青山羊(Capra hircus)和季节性发情辽宁绒山羊(C.hircus)作为实验对象,应用反转录PCR和定量PCR技术分别对两个基因的组织表达谱及繁殖轴组织中两个基因的表达差异进行检测分析。研究发现,(1)DIO2基因在所研究的24个组织都有一定量表达,DIO3基因主要在小脑、海马、下丘脑、垂体、脑桥以及卵巢和肾上腺等表达,品种间两基因组织表达谱相似;(2)济宁青山羊垂体DIO2基因表达量显著高于辽宁绒山羊(P<0.05),下丘脑、卵巢表达量也均高于辽宁绒山羊,但两品种间差异不显著(P>0.05),子宫表达量显著低于辽宁绒山羊(P<0.05);(3)对于下丘脑组织DIO3基因,则济宁青山羊表达量比辽宁绒山羊明显要高(P<0.05),而对于卵巢和子宫DIO3基因表达量,则济宁青山羊明显比辽宁绒山羊低(P<0.05),济宁青山羊垂体DIO3基因表达量低于辽宁绒山羊,但两品种间差异不显著(P>0.05)。本研究提示DIO2和DIO3基因可能与山羊季节性繁殖调控有关,研究结果可为初步揭示山羊繁殖季节性分子调控机制提供参考。As we all known, the availability of thyroid hormone within the hypothalamus is a key determinant of seasonal transitions and they convert between bioactive and inactivating type through catalyzing by type Ⅱ iodothyronine deiodinase（DIO2） and type Ⅲ iodothyronine deiodinase（DIO3）. So far,many previous studies has revealed that DIO2 and DIO3 genes might regulate seasonal reproduction in rodents（Rodentia） and sheep（Ovis aries）, but it is not clear in regulation of goat seasonality. To determine the roles of the 2 genes in goat（Capra hircus） seasonal reproduction, their spatial expression profiles and the differences ofexpression levels in hypothalamic-pituitary-gonadal axis were analyzed between Jining Grey goat（year-round estrus） and Liaoning Cashmere goat（seasonal estrus） by using RT- PCR and Real- time quantitative PCR methods, respectively. The results showed that（1） DIO2 m RNA expressed in almost all tissues studied, while DIO3 m RNA was mainly found in goat hypothalamus, pituitary, cerebellum, hippocampus, pons, and it was also determined in ovary, adrenal gland and slightly expression in goat spleen, both gene were with similar tissue profiles between two goat breeds except a small quantity of DIO3 expression in cerebral cortex in Liaoning cashmere goat.（2） DIO2 was found in all of 4 tissues detected which related to reproductive regulation and in Jining Grey goat, it expressed higher in pituitary than other tissues（P〈0.05）. For Liaoning Cashmere goat, the expressions were significant different among 4 tissues except between hypothalamus and pituitary. DIO2 m RNA expression was higher in pituitary for Jining Grey than Liaoning Cashmere goat（P〈0.05）, while that was lower in uterus than Liaoning Cashmere goat（P〈0.05） and without obvious difference in ovary and hypothalamus between 2 breeds even it appeared higher levels in Jining Grey goat（P〉0.05）.（3）In Jining Grey goat, the expression of DIO3 was the highest（P〈0.05） in hypothalamus amon

关 键 词：繁殖季节性 2型脱碘酶基因(DIO2) 3型脱碘酶基因(DIO3) 表达 山羊 

分 类 号：S831.3[农业科学—畜牧学]