基于UPLC-Q-TOF-MS技术的北青龙衣有效成分动态变化分析  

Dynamic variation of major effective components in fresh rejuvenated fruit of Juglans mandshurica based on UPLC-Q-TOF-MS

作　　者：霍金海[1,2] 孙国东 董文婷 王伟明 

机构地区：[1]黑龙江省中医药科学院中药研究所,黑龙江哈尔滨150036 [2]黑龙江中医药大学药学院,黑龙江哈尔滨150040

出　　处：《中国中药杂志》2016年第18期3379-3388,共10页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81503348);国家"重大新药创制"科技重大专项(2010ZX09102-138);哈尔滨市青年科技创新人才基金项目(2012RFOYSO42)

摘　　要：采用UPLC-Q-TOF-MS/MS技术分析不同采集时期北青龙衣有效成分动态变化规律。用Marker View软件对6个采集时期18批样品测定数据进行主成分分析,差异性化合物通过Peakview 2.0/Masterview1.0软件,依据精确质量数和同位素峰度比确定分子式,再通过对照品及数据库的二级谱图比对、裂解规律分析,结合已有文献报道,确定结构式。结果表明不同采集期萘醌类有效成分含量发生了明显的变化,鉴定或推断了北青龙衣中38个萘醌类化合物结构,随着采收时间的变化大部分萘醌类成分含量逐渐下降。该方法快速、准确,为北青龙衣的化学成分鉴定与分析提供一种新的策略,为揭示不同生长期北青龙衣有效成分动态变化规律及确定其药材合理采收期提供基础资料。The changes in effective components of Juglans mandshurica at different harvest periods were analyzed by UPLC-Q- TOF-MS/MS. Eighteen batch samples of J. mandshurica from six harvest periods were assessed by multivariate statistical analysis with Markerview software. The formula of different compounds were determined by accurate mass and isotopic abundance ratio from target screening function of Feakview 2.0/Masterviewl. 0 software. Then their structure were determined by analysis of MS/MS fragment or comparison with standard substances and references. Naphthoquinone are the major markers in samples of Juglans mandshurica from different harvest periods. Thirty-eight of naphthalenequinones were identified or inferred in J. mandshurica and contents decline gradually. UPLC-Q-TOF-MS method which develops a new strategy can identify and analyze chemical constitu- ents from J. mandshurica rapidly and accurately, main chemical constituents can be used for quality evaluation and efficacy mate- rial research. The dynamic changes in the metabolite accumulation of J. mandshurica the basic data for harvesting medicinal plants at different times.

关 键 词：液相色谱-四极杆飞行时间质谱 北青龙衣 萘醌 动态变化 

分 类 号：R284.1[医药卫生—中药学] O657.63[医药卫生—中医学]