蟾毒灵抑制NF-κB引起人食管癌细胞凋亡  

Effect of Bufalin on apoptosis in human esophageal cancer through inhibiting NF-κB

作　　者：王晶晶[1] 王巍东[2] 康小红[1] 寇小格[1] 

机构地区：[1]新乡医学院第一附属医院肿瘤科,453100 [2]新乡医学院第三附属医院输血科,453000

出　　处：《实用医学杂志》2016年第17期2784-2787,共4页The Journal of Practical Medicine

基　　金：国家自然科学基金资助项目(编号:81503414)

摘　　要：目的:观察蟾毒灵对人食管癌ECA109细胞生长的影响,探讨蟾毒灵抑制食管癌细胞抗肿瘤作用的可能机制。方法:CCK-8法测定蟾毒灵对人食管癌ECA109细胞增殖的影响;流式细胞术检测蟾毒灵对细胞凋亡的影响;Western blot法检测蟾毒灵作用于人食管癌ECA109后,NF-κBp65,ERK,P-ERK1/2及Caspase-3、Cleaved Caspase-3,PARP、Cleaved PARP蛋白表达的变化情况。结果:蟾毒灵对人食管癌ECAl09细胞有明显抑制增殖的作用,并呈现出时间和浓度依赖性;蟾毒灵能诱导ECA109细胞凋亡;蟾毒灵能使ECA109细胞中凋亡相关蛋白Cleaved Caspas-3及Cleaved PARP蛋白表达随着药物浓度的增加而明显增加,p-ERK1/2,NF-κBp65蛋白的表达随着药物浓度的增加而减少。结论 :蟾毒灵可显著抑制人食管癌ECA109细胞的增殖,促使肿瘤细胞凋亡,其作用机制可能与抑制ERK/NF-κB通路有关。Objective To observe the effect of Bufalin on controlling ECA109 cells, and to explore its potential anti-tumor mechanism. Method The effect of Bufalin on the proliferation of human esophageal cancer ECA109 cells was evaluated by CCK-8 assay and it effects on apoptosis of esophageal cancer ECA109 cells were determined by flow cytometry （FCM）. Protein expressions of NF-κBp65, ERK, PERK1/2 and Caspase-3, Cleaved Caspase-3, PARP, Cleaved PARP in esophageal cancer ECA109 cell were observed through Western blot. Results The proliferation of human esophageal cancer ECA109 cells was significantly inhibited in bufalin group in a time- and concentration-dependent manner. Bufalin can induce the apoptosis in human esophageal ECA109 cells. Results of Western blot showed the protein expressions of apoptosis-related protein Cleaved Caspas-3 and Cleaved PARP in esophageal cancer ECA109 ceils could be markedly up-regulated by Bufalin, but p-ERK1/2, NF-κBp65 in esophageal cancer ECA109 cells could be markedly down-regulated by Bufalin. Conclusion Bufalin can potently inhibit the growth of esophageal cancer ECA109 cells and the potential anti-tumor mechanism might be involved in inhibiting ERK/NF-κB signal pathway.

关 键 词：蟾毒灵 食管癌细胞 NF-ΚB 细胞凋亡 

分 类 号：R285[医药卫生—中药学]