HPLC法同时测定疏表灵颗粒中绿原酸、橙皮苷、黄芩苷的含量  

Simultaneous Determination of Chlorogenic Acid,Hesperidin and Baicalin in Shubiaoling Granule by HPLC

作　　者：李昂[1] 阚红玉[2] 张囡[1] 

机构地区：[1]天津市儿童医院,天津300134 [2]天士力制药集团股份有限公司,天津300193

出　　处：《中国药房》2016年第27期3872-3874,共3页China Pharmacy

摘　　要：目的：建立同时测定疏表灵颗粒中绿原酸、橙皮苷、黄芩苷含量的方法。方法：采用高效液相色谱法。色谱柱为SAGATri—SalC18流动相为乙腈．0．5％磷酸（梯度洗脱），流速为1．0ml／min，检测波长为320nm，柱温为30℃，进样量为10μl。结果：绿原酸、橙皮苷、黄芩苷的检测质量浓度线性范围分别为2．463-240．5μg／ml（r=0．9999）、6．577--642．3μg/ml（r=0.9998）、1．708～166．8μg／m1（r=0．9998）；精密度、稳定性、重复性试验的RSD≤1．8％；加样回收率分别为97．02％～102．40％（RSD=1．9％，n=6）、96．55％～100．20％（RSD=1．6％，n=6）、97．51％～100．70％（RSD=1．2％，n=6）。结论：该方法操作简便，结果准确、灵敏度高、重复性好，可用于疏表灵颗粒中绿原酸、橙皮苷、黄芩苷含量的同时测定。OBJECTIVE ： To establish a method for the simultaneous determination of chlorogenic acid, hesperidin and baicalin in Shubiaoling granule. METHODS： HPLC was performed on the column of SAGA Tri-Sal C18 with mobile phase of acetonitrile - 0.5% phosphoric acid （gradient elution） at a flow rate of 1.0 ml/min, detection wavelength was 320 nm, column temperature was 30 %, injection volume was 10 μl. RESULTS： The linear range was 2.463-240.5μg/ml for chlorogenic acid （r=0.999 9）, 6.577-642.3μg/ml for hesperidin （r=0.999 8） and 1.708-166.8 μg/ml for baicalin （r=0.999 8） ; RSDs of precision, stability and reproducibility tests were no more than 1.8% ; recoveries were 97.02% -102.40% （RSD=1.9% , n=6） , 96.55% -100.20% （RSD=1.6% , n=6） and 97.51% - 100.70 % （RSD = 1.2 %, n = 6）, respectively. CONCLUSIONS ： The method is simple, accurate with high sensitivity and good reprosucibility, and can be used for the simultaneous determination of chlorogenic acid, hesperidin and baicalin in Shubiaoling granule.

关 键 词：疏表灵颗粒 绿原酸 橙皮苷 黄芩苷 高效液相色谱法 

分 类 号：R917[医药卫生—药物分析学]