孕期慢性应激对子鼠情绪及海马组织中单胺类神经递质的影响  

Effects of Maternal Chronic Stress During Pregnancy on Emotion and Hippocampal Monoamine Neurotransmitters of Offspring Rats

作　　者：关素珍[1] 宁丽[2] 徐仙[2] 刘继文[2] 

机构地区：[1]新疆医科大学公共卫生学院社会医学教研室,新疆乌鲁木齐830011 [2]新疆医科大学公共卫生学院劳动卫生与环境卫生学教研室,新疆乌鲁木齐830011

出　　处：《环境与职业医学》2016年第9期849-853,共5页Journal of Environmental and Occupational Medicine

基　　金：新疆维吾尔自治区青年科学基金项目(编号:2013211B50)

摘　　要：[目的]观察大鼠孕期处于应激状态对其子代情绪的影响,从海马神经递质变化探讨其可能机制。[方法]建立孕期慢性不可预知温和应激大鼠(chronic unpredictable mild stress,CUMS)模型,采用放射免疫法测定母鼠血浆皮质酮水平,利用液体消耗实验、行为学旷场实验、悬尾实验进行子鼠情绪变化的测定,ELISA方法测定子鼠大脑海马组织中单胺类神经递质质量浓度,并分析其相关性。[结果]模型组母鼠皮质酮水平与对照组比较,差异有统计学意义(F=12.347,P=0.001),提示模型组大鼠处于应激状态。模型子鼠组的体重在PND 28和PND 42均低于对照子鼠组(P<0.05);子鼠情绪变化:模型子鼠组水平及垂直运动得分低于对照子鼠组(P<0.05);糖水消耗和1%蔗糖偏爱百分比模型子鼠组得分均低于对照子鼠组(P<0.05);悬尾实验模型子鼠组较对照子鼠组静止时间长、挣扎次数低(P<0.05)。模型子鼠组单胺类神经递质(去甲肾上腺素、多巴胺、5-羟色胺)均下降。[结论]孕期慢性应激影响子鼠情绪变化,可能与母体血浆皮质酮升高,与子鼠海马组织中枢神经递质降低有关。[Objective] To observe the impact on emotion in offspring rats following maternal stress during pregnancy and the possible mechanisms related with hippocampal monoamine neurotransmitters. [Methods] A rat model subjected to chronic unpredictable mild stress （CUMS） during pregnancy was established to measure plasma corticosterone levels of maternal rats by radioimmunoassay. Their offspring were determined for emotional responses using sucrose preference test （SPT）, open-field test （OFT）, and suspend tail test （STT）, and for levels of hippocampal monoamine neurotransmitters using ELISA for correlation analysis.[Results] An elevation was observed in the plasma corticosterone level of the rat model group compared with the control rats （F=12.347, P=0.001）, indicating that the rat model group was under stress. The body weight of the model offspring group was lower than that of the control offspring group in PND 28 and PND 42 （P 〈 0.05）. The model offspring had lower scores of horizontal movement and vertical movement than the control offspring （P 〈 0.05）. Lower consumption of sugar water and 1% sucrose preference were found in the model offspring （P 〈 0.05）. The model offspring also presented longer rest time and less struggle counts （P 〈 0.05） in the suspend tail test. Moreover, decreased levels of hippocampal monoamine neurotransmitters including norepinephrine, dopamine, and 5-hydroxytryptamine were found in the model offspring （P 〈 0.05）. [Conclusion] The emotion alteration in offspring is related to elevated maternal plasma corticosterone and decreased hippocampal monoamine neurotransmitters of offspring rats following maternal chronic stress during pregnancy.

关 键 词：孕期 应激 子鼠 情绪 海马 单胺类神经递质 去甲肾上腺素 多巴胺 5-羟色胺 

分 类 号：R114[医药卫生—卫生毒理学]